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The change and effect of Beclin 1 on cytotoxicity of ovarian cancer cells induced by MG132

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2014 01
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The change and effect of Beclin 1 on cytotoxicity of ovarian cancer cells induced by MG132
Liu Chuan1Hu Zhenhua1Tan Mingzi1Liu Baoqin2Ying Tianshu1Lin Bei1
1.Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Liaoning Shenyang 110004,China;2.Department of Biochemistry and Molecular Biology,China Medical University,Liaoning Shenyang 110001,China.
proteasome inhibitorautophagyapoptosisBeclin 1ovarian cancer
Objective:To investigate the change and the effect of Beclin 1 on cytotoxicity of ovarian cancer cells mediated by proteasome inhibitor MG132.Methods:OVCAR3 cells were treated with indicated concentrations of proteasome inhibitor MG132 for 24h,cell viability was measured using MTT assay and beclin 1 expression was detected using Western blot.OVCAR3 cells were transfected with mock or Beclin 1 eukaryotic plasmid,then treated with MG132,cell viability was measured using MTT assay; Nuclear morphology was analyzed using Hoechst 33258 staining.OVCAR3 cells were transfected with Beclin 1 specific shRNA(shBeclin 1),then treated with MG132 for 24h,the formation of acidic vacuoles were analyzed using AO staining.Results:1,2,5,10 μmol/L MG132 resulted in suppression of cell growth(P<0.05);5μmol/L and 10μmol/L MG132 reduced Beclin 1 expression at the protein levels in OVCAR3 cells(both P=0.001).Overexpression of Beclin 1 enhanced the cytotoxicity of OVCAR3 cells mediated by MG132(P=0.00089),and apoptotic morphological characteristics turned more predominant.Downregulation of Beclin 1 by shRNA demonstrated no obvious effect on accumulation of acidic vesicular organelles induced by MG132.Conclusion:MG132 reduced the Beclin 1 level in OVCAR3 cells,Beclin 1 enhanced the cytotoxicity of OVCAR3 cells mediated by MG132 in an autophagy-independent manner.


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Last Update: 2013-12-03