|Table of Contents|

Prescription form of Strengthening Qi and Clearing Stasis and Poison effect on HepG2 liver cancer cell proliferation and the influence of the nude mouse transplantation tumor growth

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2014 01
Page:
8-0011
Research Field:
Publishing date:

Info

Title:
Prescription form of Strengthening Qi and Clearing Stasis and Poison effect on HepG2 liver cancer cell proliferation and the influence of the nude mouse transplantation tumor growth
Author(s):
Zeng Puhua1; Gao Wenhui2; Pan Minqiu1; Zhu Kejian1; Jiang Yilan1; Li Yongmin1; Tang Zhen1
1.State Administration of Traditional National Key Disciplines，Affiliated Hospital,Hunan Academic University of TCM,Hunan Changsha 410006,China；2.Institute of TCM,Hunan University of Chinese Medicine,Hunan Changsha 410208,China.
Keywords:
Prescription form of SQCSP; HepG2 cells; Proliferation; transplantation tumor
PACS:
R73-36+1;R735.7
DOI:
10.3969/j.issn.1672-4992.2014.01.03
Abstract:
Objective:To investigate the effects of Prescription form of Strengthening Qi and Clearing Stasis and Poison（SQCSP）of human hepatocellular carcinoma HepG2 cells proliferation and the influence of the nude mouse transplantation tumor growth.Methods:With SQCSP drug-containing serum intervention on HepG2 cells,observing cell proliferation condition after 24,48,72 hours.Human liver transplantation tumor model,were divided into 7 groups,respectively by drug intervention such as SQCSP,astragalus root,rhizoma zedoariae,paris polyphylla,gecko,cisplatin,to observe the transplanted tumor growth for 3 weeks.Results：The dose of medicated serum group and do not contain medicine absorbance differences between serum group significantly (P＜0.05).Comparison between the high and low dose groups had significant difference (P＜0.05).Nude mice weight comparison:cisplatin group were different before and after the change compared with other groups (P＜0.05),no difference between the other groups (P＞0.05).Drug delivery to tumors before and after product comparison:tumors and different comparison,after the treatment group and the blank group were significant different (P＜0.05).All parties respectively with astragalus membranaceus group,paris polyphylla,gecko group and blank control group were significant different (P＜0.05).The whole party and cisplatin group was no significant different (P＞0.05).Conclusion：SQCSP medicated serum can inhibit the growth of human hepatocellular carcinoma HepG2 cells.The whole party group had no effect on body weight in a tumor-burdened nude mice,the whole party group has stronger role in tumor suppression compare with a single taste traditional Chinese medicine (TCM),the formula of TCM such as rhizoma zedoariae,paris polyphylla,gecko has a certain degree tumor suppression function,including rhizoma zedoariae tumor suppression effect is stronger.Formula in the main single Chinese medicine played a coordination tumor suppression effect,the whole party has the strongest inhibitory tumor effect.

References:

［1］黄继汉，黄晓晖，陈志扬，等.药理试验中动物间和动物与人体间的等效剂量换算［J］.中国临床药理学与治疗学，2004，9（9）：1069-1072.
［2］李琳霈，潘博，潘敏求.潘敏求主任医师治疗肝癌经验［J］.湖南中医杂志，2010（6）：23-25.
［3］金炜东，陈孝平，蔡红娇.重楼提取物对HepG2细胞的毒性作用［J］.华中科技大学学报，2006，35（1）:103-105.
［4］杨丽华，杨金霞，王学美．鲜壁虎不同部位提取液对鼠肝癌体内外抑制作用［J］.世界华人消化杂志，2007，15 (35)：3734-3737.
［5］曾普华，郜文辉，潘敏求，等.益气化瘀解毒方加减联合鸦胆子油乳经血管介入治疗中晚期原发性肝癌的临床研究［J］.辽宁中医杂志，2013，40（1）：19-20.
［6］郜文辉，曾普华，滕久祥，等.益气化瘀解毒方含药血清对鸡胚尿囊膜血管生成的影响［J］.中华中医药学刊，2013，31（1）：19-20.

Memo

Memo:
国家自然科学基金资助项目（编号：81001522）；湖南省自然科学基金课题（编号：13JJ4138）；首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金资助项目（编号：2010-F10）
Last Update: 2013-12-03