|Table of Contents|

The regulation of Wnt3a on the cell growth in non-small-cell lung cancer cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2014 01
Page:
1-0003
Research Field:
Publishing date:

Info

Title:
The regulation of Wnt3a on the cell growth in non-small-cell lung cancer cell lines
Author(s):
Li Chunyan1; Sheng Linjun2; Song Gongru3
1.Center of the Laboratory Technology and Experimental Medicine,China Medical University,Liaoning Shenyang 110001,China；2.Grade 2006,Department of Biotechnology,China Medical University,Liaoning Shenyang 110001,China；3.Grade 2007,Department of Biotechnology,China Medical University,Liaoning Shenyang 110001,China.
Keywords:
NSCLC; Wnt signaling pathway; cell cycle; cell growth
PACS:
R730.23;R734.2
DOI:
10.3969/j.issn.1672-4992.2014.01.01
Abstract:
Objective:To approach the molecular mechanism of Wnt signaling pathway in non-small-cell lung cancer(NSCLC),and the effect of recombinant human Wnt3a on NSCLC cell lines.Methods:H460 and A549 cell lines were treated with recombinant human Wnt3a.The alteration of the cell numbers,colony forming in soft-agar and cell cycle were observed.Results:The treatment of recombinant human Wnt3a significantly increased cell numbers ［（4.26±0.26)×105/ml vs (2.42±0.44)×105/ml in A549,P＜0.01；(3.98±0.21)×105/ml vs (2.07±0.10)×105/ml in H460，P＜0.01］and the efficiency of colony forming［（1.57±0.11)% vs (1.03±0.08)% in A549,P＜0.01；(1.53±0.11)% vs (0.79±0.10)% in H460，P＜0.01］.The flow cytometric analysis showed that the cells of G0/G1 phase declined and the cells of S phase rose after the treatment of recombinant human Wnt3a.Conclusion:As an activator of canonical Wnt signaling,recombinant human Wnt3a may promote cell growth through its regulation to cell cycle in NSCLC cell lines.

References:

［1］Pongracz JE,Stockley RA.Wnt signaling in lung development and diseases［J］.Respiratory Res,2006,7:15-24.
［2］Akiri G,Cherian MM,Vijayakumar S,et al.Wnt pathway aberrations including autocrine Wnt activation occur at high frequency in human non-small-cell lung carcinoma［J］.Oncogene,2009,28:2163-2172.
［3］Nakashima N,Huang CL,Liu D,et al.Intratumoral Wnt1 expression affects survivin gene expression in non-small cell lung cancer［J］.Int J Oncol,2010,37:687-694.
［4］Huang Y,Liu G,Zhang B,et al.Wnt-5a regulates proliferation in lung cancer cells［J］.Oncol Rep,2010,23:177-181.
［5］Wei Q,Zhao Y,Yang ZQ,et al.Dishevelled family proteins are expressed in non-small cell lung cancer and function differentially on tumor progression［J］.Lung Cancer,2008,62:181-192.
［6］Kim J,You L,Xu Z,et al.Wnt inhibitory factor inhibits lung cancer cell growth［J］.J Thorac Cardiovasc Surg,2007,133:733-737.
［7］Yue W,Sun Q,Dacic S,et al.Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer［J］.Carcinogenesis,2008,29:84-92.
［8］Yang TM,Leu SW,Li JM,et al.WIF-1 promoter region hypermethylation as an adjuvant diagnostic marker for non-small cell lung cancer-related malignant pleural effusions［J］.J Cancer Res Clin Oncol,2009,135:919- 924.
［9］Xu X,Sun PL,Li JZ,et al.Aberrant Wnt1/β-Catenin expression is an independent poor prognostic marker of non-small cell lung cancer after surgery［J］.J Thorac Oncol,2011,6(4):716-724.
［10］Chunyan Li,Ying Zhang,Yao Lu,et al.Evidence of the cross talk between Wnt and Notch signaling pathways in non-small-cell lung cancer (NSCLC):Notch3- siRNA weakens the effect of LiCl on the cell cycle of NSCLC cell lines［J］.J Cancer Res Clin Oncol,2011,137(5):771-778.
［11］Kikuchi A，Yamamoto H，Kishida S．Multiplicity of the interactions of Wnt proteins and their receptors［J］．Cell Signal，2007，19(4)：659-671．
［12］Boland GM,Perkins G,Hall DJ,et al.Wnt3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cell［J］.J Cell Biochem，2004，93(6):1210-1230.
［13］Van de Wetering M,Sancho E,Verweij C,et al.The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells［J］.Cell,2002，111:241-250.
［14］Lim JH,Chun YS,Park JW.Hypoxia-inducible factor-1alpha obstructs a Wnt signaling pathway by inhibiting the hARD1-mediated activation of beta-catenin［J］.Cancer Res,2008,68:5177-5184.
［15］Tang Y,Simoneau AR,Liao WX,et al.WIF1,a Wnt pathway inhibitor,regulates SKP2 and c-myc expression leading to G1 arrest and growth inhibition of human invasive urinary bladder cancer cells［J］.Mol Cancer Ther,2009,8(2):458-468.
［16］Wang X,Meng X,Sun X,et al.Wnt/beta-catenin signaling pathway may regulate cell cycle and expression of cyclin A and cyclin E protein in hepatocellular carcinoma cells［J］.Cell Cycle,2009，8:1567-1570.

Memo

Memo:
辽宁省自然科学基金项目（编号：201102258）；教育部留学回国人员科研启动基金项目（编号：2009-1001）
Last Update: 2013-12-03