|Table of Contents|

Water extract from patrinia scabiosaefolia inhibits the proliferation,migration and invasion of glioma cells by regulating circ_0000936/miR-665

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
1017-1024
Research Field:
Publishing date:

Info

Title:
Water extract from patrinia scabiosaefolia inhibits the proliferation,migration and invasion of glioma cells by regulating circ_0000936/miR-665
Author(s):
ZHANG Zhengping1; LI Kunzheng1; WU Binjie1; LIU Xi1; JU Hu1; ZHANG Xiaolu2
1.Department of Neurosurgery,Affiliated Hospital of Qinghai University,Qinghai Xining 810000,China;2.Department of Neurosurgery,Wuxi People's Hospital Affiliated to Nanjing Medical University,Jiangsu Wuxi 214023,China.
Keywords:
patrinia scabiosaefolia; glioma; circ_0000936; miR-665; cell proliferation; migration; invasion
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2024.06.006
Abstract:
Objective:To explore the effect of water extract from patrinia scabiosaefolia on the biological behavior of glioma cells and its possible mechanism.Methods:Thirty-one cases of glioma tissue from February 2019 to January 2020 who underwent surgical resection and were pathologically confirmed in our hospital were collected.qRT-PCR method was used to detect the expression of circ_0000936 and miR-665 in normal brain tissue and glioma tissue.Pearson method was used to analyze the correlation between circ_0000936 and miR-665 expression in glioma tissues.Human glioma cells LN229 were cultured in vitro,and different concentrations of patrinia scabiosaefolia (0.5 mg/mL,1.0 mg/mL,2.0 mg/mL) were treated with LN229 cells.si-NC and si-circ_0000936 were transfected into LN229 cells respectively.After pcDNA-circ_0000936 was transfected into LN229 cells,2.0 mg/mL patrinia scabiosaefolia was added to treat the cells.CCK-8,plate clone formation experiment,Transwell chamber experiment and flow cytometry were used to detect cell proliferation,colony formation,migration,invasion and apoptosis.The dual-luciferase reporter assay was used to detect the targeting relationship between circ_0000936 and miR-665.Western blot method was used to detect the expression of Bax and Bcl-2 protein.Results:The expression of circ_0000936 in glioma tissue was higher than that in normal brain tissue (P＜0.05),while the expression of miR-665 was lower than that in normal brain tissue (P＜0.05).circ_0000936 and miR-665 were negatively correlated (r=-0.980,P＜0.001).Patrinia scabiosaefolia could reduce cell viability,clone formation rate and could reduce the protein level of Bcl-2 and the expression of circ_0000936 in a concentration-dependent manner (P＜0.05),and the number of migration and invasion cells was reduced (P＜0.05),while the rate of apoptosis,the protein level of Bax and the expression of miR-665 were increased (P＜0.05).After transfection with si-circ_0000936,the expression of miR-665 was increased (P＜0.05),and cell viability,clone formation rate and the protein level of Bcl-2 were decreased (P＜0.05),and the number of migration and invasion cells were decreased (P＜0.05),but apoptosis rate and the protein level of Bax were increased (P＜0.05).Transfection of pcDNA-circ_0000936 could reverse the effect of patrinia scabiosaefolia on the biological behavior of LN229 cells.Conclusion:Water extract from patrinia scabiosaefolia could induce apoptosis of glioma cells,and inhibit the proliferation,migration and invasion by regulating circ_0000936/miR-665.

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青海大学青年科研基金项目（编号：2020-QYY-6）
Last Update: 1900-01-01