|Table of Contents|

LncRNA GATA3-AS1 inhibits the proliferation,migration and invasion of cervical cancer cells by regulating the miR-362-3p/FABP5 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
1009-1016
Research Field:
Publishing date:

Info

Title:
LncRNA GATA3-AS1 inhibits the proliferation,migration and invasion of cervical cancer cells by regulating the miR-362-3p/FABP5 axis
Author(s):
LUO Jianwei1; HUANG Hongke2; HU Yanli3
1.College of Traditional Chinese Medicine Health and Wellness，Leshan Vocational and Technical College,Sichuan Leshan 614000，China;2.School of Biomedical Sciences，Leshan Vocational and Technical College，Sichuan Leshan 614000，China;3.Department of Gynecology，Chongqing University Qianjiang Hospital，Chongqing 400030，China.
Keywords:
long non-coding RNA GATA3 antisense RNA 1; microRNA-362-3p; cervical cancer; proliferation; transfer
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.06.005
Abstract:
Objective:To investigate the impacts of long non-coding RNA GATA3 antisense RNA 1 (lncRNA GATA3-AS1) on the malignant biological behavior of cervical cancer cells by regulating the expression of microRNA-362-3p (miR-362-3p).Methods:qRT-PCR was applied to detect the expression of lncRNA GATA3-AS1,miR-362-3p,and FABP5 in cervical cancer cells.Double Luciferase reporter gene experiment was applied to verify the targeting relationship between lncRNA GATA3-AS1 and miR-362-3p,and the targeting relationship between miR-362-3p and FABP5.Cells were divided into pcDNA-NC group,pcDNA-GATA3-AS1 group,si-NC group,si-GATA3-AS1 group,si-GATA3-AS1+inhibitor NC group,si-GATA3-AS1+miR-362-3p inhibitor group,miR-NC group,miR-362-3p mimics group,miR-362-3p mimics+pcDNA-NC group,and miR-362-3p mimics+pcDNA FABP5 group.Western blot was applied to detect protein expression.EdU method was applied to detect cell proliferation.Transwell was applied to detect cell migration and invasion.Results:In cervical cancer cell lines,GATA3-AS1 and FABP5 were highly expressed,while miR-362-3p was low expressed,HeLa cells were selected for subsequent experiments.Double Luciferase reporter gene experiment showed that lncRNA GATA3-AS1 was targeted to miR-802,miR-362-3p was targeted to FABP5.Compared with the pcDNA-NC group,the EdU positive rate,migration and invasion,and the expression of MMP-2 and MMP-9 in Hela cells in the pcDNA-GATA3-AS1 group were obviously increased (P＜0.05).Compared with the si-NC group,the EdU positive rate,migration and invasion,and the expression of MMP-2 and MMP-9 in HeLa cells in the si-GATA3-AS1 group obviously decreased (P＜0.05).Inhibiting the expression of miR-362-3p or overexpressing FABP5 was able to obviously reverse the inhibitory effects of silencing GATA3-AS1 or overexpressing miR-362-3p on the proliferation,migration,and invasion of HeLa cells.Conclusion:Silencing GATA3-AS1 can targetingly up-regulate the expression of miR-362-3p,inhibit the expression of FABP5,and inhibit the proliferation,migration,and invasion of cervical cancer HeLa cells.

References:

［1］ VIVEROS-CARRENO D,FERNANDES A,PAREJA R.Updates on cervical cancer preventions［J］.Int J Gynecol Cancer,2023,33(3):394-402.
［2］ SCHUBERT M,BAUERSCHLAG DO,MUALLEM MZ,et al.Challenges in the diagnosis and individualized treatment of cervical cancer［J］.Medicina (Kaunas),2023,59(5):925.
［3］ 白小英,曹蕾,张卫霞,等.LncRNA SNHG1通过miR-101-3p/MYLIP轴调节宫颈癌细胞增殖、凋亡和迁移的研究［J］.中国计划生育和妇产科,2023,15(4):38-43,60,113. BAI XY,CAO L,ZHANG WX,et al.LncRNA SNHG1 regulates the proliferation,apoptosis and migration of cervical cancer cells through the miR-101-3p/MYLIP axis［J］.Chinese Journal of Family Planning and Gyneocotokolony,2023,15(4):38-43,60,113.
［4］ HOU YM,WANG XP,SHEN CC,et al.Cervical carcinoma progression is aggravated by lncRNA ZNF281 by binding KLF15［J］.Eur Rev Med Pharmacol Sci,2021,25(18):5610-5618.
［5］ ZHANG M,WANG N,SONG P,et al.LncRNA GATA3-AS1 facilitates tumour progression and immune escape in triple-negative breast cancer through destabilization of GATA3 but stabilization of PD-L1［J］.Cell Prolif,2020,53(9):e12855-e12868.
［6］ LIU YX,YUAN S,LIU XJ,et al.LncRNA GATA3-AS1 promoted invasion and migration in human endometrial carcinoma by regulating the miR-361/ARRB2 axis［J］.J Mol Med (Berl),2022,100(9):1271-1286.
［7］ ALI SYEDA Z,LANGDEN SSS,MUNKHZUL C,et al.Regulatory mechanism of microrna expression in cancer［J］.Int J Mol Sci,2020,21(5):1723-1741.
［8］ WANG D,WANG H,LI Y,et al.MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma［J］.Biosci Rep,2018,3 8(3):1-9.
［9］ DOGHISH AS,ALI MA,ELYAN SS,et al.miRNAs role in cervical cancer pathogenesis and targeted therapy:Signaling pathways interplay［J］.Pathol Res Pract,2023,244(5):154386-154398.
［10］ CHEN P,ZHANG W,CHEN Y,et al.Comprehensive analysis of aberrantly expressed long non-coding RNAs,microRNAs,and mRNAs associated with the competitive endogenous RNA network in cervical cancer［J］.Mol Med Rep,2020,22(1):405-415.
［11］ LIU Y,XU G,LI L.LncRNA GATA3-AS1-miR-30b-5p-Tex10 axis modulates tumorigenesis in pancreatic cancer［J］.Oncol Rep,2021,45(5):59-69.
［12］ LIN S,ZHAO M,LV Y,et al.The lncRNA GATA3-AS1/miR-495-3p/CENPU axis predicts poor prognosis of breast cancer via the PLK1 signaling pathway［J］.Aging (Albany NY),2021,13(10):13663-13679.
［13］ CHEN X,XIE L,QIAO K,et al.The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer［J］.Aging (Albany NY),2023,15(3):689-704.
［14］ AZEVEDO MARTINS JM,RABELO-SANTOS SH,DO AMARAL WESTIN MC,et al.Tumoral and stromal expression of MMP-2,MMP-9,MMP-14,TIMP-1,TIMP-2,and VEGF-A in cervical cancer patient survival:a competing risk analysis［J］.BMC Cancer,2020,20(1):660-671.
［15］ 曹琼,代爱军,李宜川,等.miRNA-1靶向DDX5抑制食管癌细胞增殖、迁移及侵袭［J］.现代肿瘤医学,2020,28(05):702-705. CAO Qiong,DAI Aijun,LI Yichuan,et al.miRNA-1 inhibits proliferation，migration，and invasion of esophageal cancer cells by targeting DDX5［J］.Modern Oncology,2020,28(05):702-705.
［16］ 李永红,刘伟仪,李伟,等.miRNA-184在胶质瘤组织中的表达及其对胶质瘤细胞增殖和凋亡的影响［J］.现代肿瘤医学,2020,28(14):2385-2389. LI Yonghong,LIU Weiyi,LI Wei,et al.Expression of miRNA-184 in glioma tissues and its effect on glioma cells proliferation and apoptosis［J］.Modern Oncology,2020,28(14):2385-2389.
［17］ LI C,YANG Y,WANG H,et al.miR-362-3p suppresses ovarian cancer by inhibiting LRP8［J］.Transl Oncol,2022,15(1):101284-101293.
［18］ ASSIRI AA,MOURAD N,SHAO M,et al.MicroRNA 362-3p reduces hERG-related current and inhibits breast cancer cells proliferation［J］.Cancer Genomics Proteomics,2019,16(6):433-442.
［19］ YANG S,ZHANG X,SUN Y,et al.MicroRNA-362-3p inhibits migration and invasion via targeting BCAP31 in cervical cancer［J］.Front Mol Biosci,2020,7(1):107-118.
［20］ ZHANG Y,LUO M,CUI X,et al.Long noncoding RNA NEAT1 promotes ferroptosis by modulating the miR-362-3p/MIOX axis as a ceRNA［J］.Cell Death Differ,2022,29(9):1850-1863.
［21］ XIE B,WANG Z,LI T,et al.LncRNA MALAT1 inhibits the proliferation and invasiveness of laryngeal squamous cell carcinoma Hep-2 cells by modulating miR-362-3p［J］.Am J Transl Res,2022,14(6):3729-3740.
［22］ WANG W,JIA HL,HUANG JM,et al.Identification of biomarkers for lymph node metastasis in early-stage cervical cancer by tissue-based proteomics［J］.Br J Cancer,2014,110(7):1748-1758.

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