|Table of Contents|

Mechanism of action and preliminary validation of artemisinin against gastric cancer based on network pharmacology and molecular docking studies

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
993-1002
Research Field:
Publishing date:

Info

Title:
Mechanism of action and preliminary validation of artemisinin against gastric cancer based on network pharmacology and molecular docking studies
Author(s):
HUANG Xiaomin; ZHAO Xuhui; MA Taomei; DA Dezhuan; LI Hongling
The First School of Clinical Medicine,Gansu University of Chinese Medicine，Gansu Lanzhou 730000,China.
Keywords:
gastric cancer; artemisinin; network pharmacology; molecular docking; experimental validation
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2024.06.003
Abstract:
Objective:To investigate the potential mechanisms of artemisinin in the treatment of gastric cancer using network pharmacology and experimental validation.Methods:The targets of artemisinin were screened using Chinese Medicine Systems Pharmacology Database and Disease Target Database.A network diagram of "Drug-Disease-Target" was constructed using Cytoscape software.and a protein-protein interaction (PPI) network was constructed using the String database.The intersecting genes were subjected to Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis using the DAVIDA database，resulting in the construction of a network of active ingredients-targets-key pathways.Molecular docking was performed using AutoDock Vina，and binding affinities were calculated.The mRNA and protein expression levels of core targets were analyzed using databases such as GEPIA and HPA.Results:Network analysis revealed ABCB1，HSP90AA1,and VEGFA as the three core targets.Functional enrichment analysis showed that these target genes exerted a therapeutic effect on gastric cancer through the PI3K-Akt signaling pathway.In vitro experiments confirmed the inhibitory effect of artemisinin on the proliferation and migration of gastric cancer cells,and downregulation of the expression of core target genes ABCB1,HSP90AA1,and VEGFA,which may be associated with the PI3K-Akt signaling pathway.Conclusion:This study elucidated the potential targets and molecular mechanisms of artemisinin in the treatment of gastric cancer,providing new insights and clues for traditional Chinese medicine approaches to gastric cancer therapy.

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Memo

Memo:
甘肃省人民医院国家级科研项目（编号：19SYPYA-3）;甘肃省研发计划项目（编号：2021-0203-SFC-0117）
Last Update: 1900-01-01