|Table of Contents|

Bufalin inhibits the polarization of M2 macrophages induced by hypoxic drug-resistant cells and reverses drug resistance in colon cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
987-993
Research Field:
Publishing date:

Info

Title:
Bufalin inhibits the polarization of M2 macrophages induced by hypoxic drug-resistant cells and reverses drug resistance in colon cancer
Author(s):
TANG Donghao1; 2; 3; WANG Jie1; 2; 3; JIA Linlin1; JIANG Haopeng1; XIA Qi1; CHEN Jia1; CAO Saiya1; CHEN Jinbao1; SI Xianke1
1.Putuo Hospital，Shanghai University of Traditional Chinese Medicine，Shanghai 200062，China；2.Department of General Surgery,Shanghai Putuo Central School of Clinical Medicine，Anhui Medical University，Shanghai 200062，China;3.The Fifth School of Clinical Medicine，Anhui Medical University，Anhui Hefei 230032,China.
Keywords:
hypoxia; bufalin; M2 macrophages; colon cancer; drug resistance
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.06.002
Abstract:
Objective:To investigate the effect of bufalin (BU) on common colon cancer cells by inhibiting the polarization of M2 macrophages induced by drug-resistant colon cancer cells in a hypoxic microenvironment.Methods:It involved adding cobalt chloride (CoCl2) to the medium to simulate a hypoxic environment，and using phorbol 12-myristate 13-acetate (PMA) to induce the differentiation of human monocyte THP-1 into M0 macrophages.The conditioned medium of drug-resistant colon cancer cells under hypoxic conditions，common colon cancer cells，and BU acting on drug-resistant colon cancer cells were collected and then put into M0 macrophages.The expression of M2 macrophage polarization markers was detected using flow cytometry，Realtime PCR，and ELISA experiments.The effects of the supernatant after basal-induced polarization of M2 macrophages on common colon cancer were evaluated using CCK-8 and Western blot experiments.Results:In a hypoxic environment，drug-resistant colon cancer cells CM promoted the increase of M2 macrophage markers IL-10，TGF-β，CD11b，and CD206 (P＜0.01,P＜0.05) compared to common colon cancer cells.The supernatant of polarized macrophages increased the expression of P-gp and Bcl-2 in common colon cancer cells while decreasing the expression of Bax and sensitivity to oxaliplatin.After BU treatment on drug-resistant colon cancer cells，the expression of M2 macrophage markers IL-10，TGF-β，CD11b，and CD206 decreased (P＜0.01，P＜0.05).Furthermore，the expression of P-gp and Bcl-2 decreased，while the expression of Bax increased，leading to increased sensitivity to OXA.Conclusion:In hypoxic condition，colon cancer drug-resistant cell CM promotes the polarization of M2 macrophages，and bufalin can reverse colon cancer drug resistance by regulating the process of M2 macrophage polarization.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.81973625);国家自然科学基金面上项目（编号：81973625）;上海市启明星项目（编号：22YF1441400）；安徽医科大学研究生科研与实践创新项目资助（编号：YJS20230098)
Last Update: 1900-01-01