|Table of Contents|

USP11 promotes invasion and metastasis of hepatocellular carcinoma cells through regulating VEGF expression and angiogenesis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
983-987
Research Field:
Publishing date:

Info

Title:
USP11 promotes invasion and metastasis of hepatocellular carcinoma cells through regulating VEGF expression and angiogenesis
Author(s):
DU Baoying1; ZHANG Sheng2
1.Department of Hepatobiliary Pancreas Surgery;2.Department of Pathology，Hubei Cancer Hospital，Tongji Medical College，Huazhong University of Science and Technology，Hubei Wuhan 430079,China.
Keywords:
hepatocellular carcinoma; deubiquitination enzyme 11; VEGF; angiogenesis; invasion and metastasis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.06.001
Abstract:
Objective:To explore the mechanism of deubiquitination enzyme 11 (USP11) regulating VEGF and angiogenesis to promote invasion and metastasis of hepatocellular carcinoma (HCC) cells.Methods:Real-time PCR was used to detect the effect of USP11 on the expression level of VEGF gene in HCC cells.The effect of USP11 on the secretion of VEGF protein in HCC cells was detected by Elisa.Vascular endothelial cells HUVECs were treated with supernatant secreted by HCC cells in control and USP11 knockdown group respectively，and transwell matrix gel assay was used to detect the migration ability of vascular endothelial cells.The proliferation ability of vascular endothelial cells was detected by CCK-8 assay and the angiogenesis ability of vascular endothelial cells was detected by tubule formation assay.Results:Knockdown of USP11 could decrease the gene expression level of VEGF in HCC cells (P＜0.01)，and inhibit the secretion of VEGF protein (P＜0.001).Compared with the control group，the proliferation ability of vascular endothelial cells was weaker in USP11 knocked down group (P＜0.01)，and the angiogenesis ability was much lower than that of the control group.HCC cells and vascular endothelial cells were co-cultured in the two groups.Matrix gel experiment showed that the migration ability of vascular endothelial cells was decreased in USP11 knocked down group (P＜0.01).Conclusion:USP11 increases the gene transcription and protein expression level of VEGF，enhances the proliferation，migration and angiogenesis ability of vascular endothelial cells，thus promoting the invasion and metastasis of HCC cells.

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Memo

Memo:
National Natural Science Foundation of China(No.81871910);国家自然科学基金面上项目（编号：81871910）
Last Update: 1900-01-01