|Table of Contents|

NDUFA4 promotes the proliferation,migration and invasion of ovarian cancer SKOV3 cells by enhancing mitochondrial activation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 06
Page:
1024-1032
Research Field:
Publishing date:

Info

Title:
NDUFA4 promotes the proliferation,migration and invasion of ovarian cancer SKOV3 cells by enhancing mitochondrial activation
Author(s):
MENG Huanran1; DONG Hui2; FENG Fanfan3; MA Pengzhu3; TIAN Xuemin3; SHI Lulu3; YANG Caihong1
1.Obstetrics and Gynecology,General Hospital of Ningxia Medical University,Cardio-cerebrovascular Hospital,Ningxia Yinchuan 750004,China;2.General Hospital of Ningxia Medical University,Ningxia Yinchuan 750004,China;3.Ningxia Medical University,Ningxia Yinchuan 750004,China.
Keywords:
ovarian cancer SKOV3 cell line; NDUFA4 gene; mitochondrial activation; oxidative phosphorylation
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2024.06.007
Abstract:
Objective:To investigate the effect of mitochondrial activation level mediated by NDUFA4 on proliferation,migration and invasion of ovarian cancer SKOV3 cells.Methods:NDUFA4 overexpression and silencing models of SKOV3 cells were established by lentivirus technique.Cell proliferation was detected by CCK-8,cell migration was detected by scratch,cell invasion was detected by Transwell,mitochondrial membrane potential and apoptosis were detected by flow cytometry.The mitochondrial DNA copy number,MT-ND1,MT-ND5,MT-CYB,SDHA,PGC-1α,NRF1 and mTFA mRNA expressions were detected by Real-time PCR.The morphology of mitochondria was observed by electron microscope.The activity level of Complex IV was determined by spectrophotometry.The expressions of PGC-1α,NRF1 and mTFA were detected by Western blot.Results:NDUFA4 was overexpressed and silenced in SKOV3 cells.Overexpression of NDUFA4 increased cell proliferation,invasion and migration (P＜0.05),up-regulated mtDNA copy number,and up-regulated mRNA levels of MT-ND1,MT-ND5,MT-CYB,SDHA,PGC-1α,NRF1 and mTFA (P＜0.05).The protein levels of PGC-1α,NRF1 and mTFA were up-regulated (P＜0.05),and the mitochondrial membrane potential and Complex IV activity levels were up-regulated (P＜0.05),mitochondria are pluralistic and normal.The silencing of NDUFA4 was opposite，mitochondria vacuolated.Conclusion:NDUFA4 may play a role in promoting cancer in SKOV3 cells by enhancing mitochondrial activation and oxidative phosphorylation.

References:

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Last Update: 1900-01-01