|Table of Contents|

Expression of GNPDA1 in epithelial ovarian cancer and its prognostic significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
900-907
Research Field:
Publishing date:

Info

Title:
Expression of GNPDA1 in epithelial ovarian cancer and its prognostic significance
Author(s):
LEI Shunmei1; YIN Fuqiang2; KE Yao2; ZHANG Han1; WEI Dan1; SU Yuting2; XIA Lianping2; CHEN Xiaoying2; LIU Xia1
1.School of Preclinical Medicine,Centre for Translational Medicine,Key Laboratory of Longevity and Ageing Related Disease of Chinese Ministry of Education，Guangxi Medical University,Guangxi Nanning 530021,China;2.Life Science Institute,Key Laboratory of Early Prevention and Treatment of Regional High-incidence Tumors,Ministry of Education,Guangxi Medical University，Guangxi Nanning 530021,China.
Keywords:
GNPDA1; ovarian cancer; ferroptosis; prognosis
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2024.05.020
Abstract:
Objective:To investigate the correlations between glucosamine-6-phosphate deaminase 1(GNPDA1) expression with prognosis of ovarian cancer(OC),and to elucidate the potential molecular mechanism of GNPDA1 affecting the progression and prognosis of OC.Methods:The expression of GNPDA1 in ovarian tissues was detected by immunohistochemical streptavidin-peroxidase-biotin connection method(IHC).Pearson's χ2 test(2-sided) was used to analyze the correlations between GNPDA1 and clinicopathological factors.Kaplan-Meier plot was used to calculate the probability of survival and significance.The functional clustering of biological pathways enriched from co-expressed genes of GNPDA1 was used to discuss the possible molecular mechanisms that GNPDA1 may lead to poor prognosis in OC.Results:IHC results of 224 OC tissues showed that the protein expression of GNPDA1 was significantly increased in OC tissues compared with in normal tissues(P＜0.05).The over-expression of GNPDA1 protein was significantly associated with overall survival(OS) in 150 OC tissues(P=0.001).Moreover,the overexpression of GNPDA1 mRNA predicted poor OS and progression-free survival(PFS) in OC patients according to Kaplan-Meier plotter(P＜0.001).Functional clustering of biological pathways enriched from the co-expressed genes of GNPDA1 in OC tissues reveled that GNPDA1 potentially involved in regulation of OC via multiple pathways such as ferroptosis,lysosome and metabolic pathways.Further analysis showed that 18 GNPDA1 positively co-expressed genes were enriched in ferroptosis pathway in 1 815 OC tissues,and 11 of them were significantly associated with poor prognosis from KM plotter(P＜0.05).The combined overexpression of GNPDA1 and 11 ferroptosis-related genes showed better prognosis potential for OS and PFS than any of the individually tested genes(P＜0.001).Conclusion:Overexpression of GNPDA1 potentially involved in prognosis of OC via ferroptosis pathways,suggested that GNPDA1 may be a new biomarker and a therapeutic for OC.

References:

［1］SIMINIAK N,CZEPCZYNSKI R,ZABOROWSKI MP,et al.Immunotherapy in ovarian cancer［J］.Arch Immunol Ther Exp(WARSZ),2022,70(1):19.
［2］SASAMOTO N,ELIAS KM.Early detection of ovarian cancer［J］.Cold Spring Harb Perspect Med,2023,8:a041337.
［3］RADU MR,PRADATU A,DUICA F,et al.Ovarian cancer:Biomarkers and targeted therapy［J］.Biomedicines,2021,9(6):693.
［4］ALVAREZ-A ORVE LI,ALONZO DA,MORA-LUGO R,et al.Allosteric kinetics of the isoform 1 of human glucosamine-6-phosphate deaminase［J］.Biochim Biophys Acta,2011,1814(12):1846-1853.
［5］LI D,CHENG X,ZHENG W,et al.Glucosamine-6-phosphate isomerase 1 promotes tumor progression and indicates poor prognosis in hepatocellular carcinoma［J］.Cancer Management and Research,2020,12:4923-4935.
［6］GU J,ZHANG J,HUANG W,et al.Activating miRNA-mRNA network in gemcitabine-resistant pancreatic cancer cell associates with alteration of memory CD4+T cells［J］.Annals of Translational Medicine,2020,8(6):279.
［7］HE YJ.Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester［J］.World Journal of Gastroenterology,2014,20(33):11840.
［8］Cancer Genome Atlas Research Network.Integrated genomic analyses of ovarian carcinoma［J］.Nature,2011,474(7353):609-615.
［9］CERAMI E,GAO J,DOGRUSOZ U,et al.The cBio cancer genomics portal:An open platform for exploring multidimensional cancer genomics data［J］.Cancer Discovery,2012,2(5):401-404.
［10］REMMELE W,STEGNER HE.Recommendation for uniform definition of an immunoreactive score(IRS) for immunohistochemical estrogen receptor detection(ER-ICA) in breast cancer tissue［J］.Pathologe,1987,8(3):138-140.
［11］YIN F,YI S,WEI L,et al.Microarray-based identification of genes associated with prognosis and drug resistance in ovarian cancer［J］.Cell Biochem,2019,120(4):6057-6070.
［12］DONG C,YIN F,ZHU D,et al.NCALD affects drug resistance and prognosis by acting as a ceRNA of CX3CL1 in ovarian cancer［J］.Cell Biochem,2020,121(11):4470-4483.
［13］LIAO Y,WANG J,JAEHNIG EJ,et al.WebGestalt 2019:Gene set analysis toolkit with revamped UIs and APIs［J］.Nucleic Acids Research,2019,47(W1):W199-W205.
［14］YUAN X,ZHOU Q,ZHANG F,et al.Identification of immunity-and ferroptosis-related genes for predicting the prognosis of serous ovarian cancer［J］.Gene,2022,838:146701.
［15］SHEVCHENKO V,HOGBEN M,EKONG R,et al.The human glucosamine-6-phosphate deaminase gene:cDNA cloning and expression,genomic organization and chromosomal localization［J］.Gene,1998,216(1):31-38.
［16］WOLOSKER H,KLINE D,BIAN Y,et al.Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity［J］.Faseb J,1998,12(1):91-99.
［17］ZHOU W,ZHANG S,CAI Z,et al.A glycolysis-related gene pairs signature predicts prognosis in patients with hepatocellular carcinoma［J］.Peer J,2020,8:e9944.
［18］XIA R,TANG H,SHEN J,et al.Prognostic value of a novel glycolysis-related gene expression signature for gastrointestinal cancer in the Asian population［J］.Cancer Cell International,2021,21(1):154-170.
［19］YANG WH,HUANG Z,WU J,et al.A TAZ-ANGPTL4-NOX2 axis regulates ferroptotic cell death and chemoresistance in epithelial ovarian cancer［J］.Mol Cancer Res,2020,18(1):79-90.
［20］HUANG Y,HUANG J,HUANG Y,et al.TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression［J］.Am J Cancer Res,2020,10(1):131-147.
［21］DAI T,LI J,LU X,et al.Prognostic role and potential mechanisms of the ferroptosis-related metabolic gene signature in hepatocellular carcinoma［J］.Pharmgenomics Pers Med,2021,14:927-945.
［22］YOUSUF U,SOFI S,MAKHDOOMI A,et al.Identification and analysis of dysregulated fatty acid metabolism genes in breast cancer subtypes［J］.Med Oncol,2022,39(12):256.
［23］WANG D,WEI G,MA J,et al.Identification of the prognostic value of ferroptosis-related gene signature in breast cancer patients［J］.BMC Cancer,2021,21(1):645-656.
［24］DU X,ZHANG Y.Integrated analysis of immunity-and ferroptosis-related biomarker signatures to improve the prognosis prediction of hepatocellular carcinoma［J］.Front Genet,2020,11:614888.
［25］LIU Y,XU Z,JIN T,et al.Ferroptosis in low-grade clioma:A new marker for diagnosis and prognosis［J］.Med Sci Monit,2020,26:e921947.
［26］CHEN X,YAN L,JIANG F,et al.Identification of a ferroptosis-related signature associated with prognosis and immune infiltration in adrenocortical carcinoma［J］.Int J Endocrinol,2021,2021:4654302.
［27］MA LL,LIANG L,ZHOU D,et al.Tumor suppressor miR-424-5p abrogates ferroptosis in ovarian cancer through targeting ACSL4［J］.Neoplasma,2021,68(1):165-173.
［28］YANG L,TIAN S,CHEN Y,et al.Ferroptosis-related gene model to predict overall survival of ovarian carcinoma［J］.J Oncol,2021,2021:6687391.
［29］CHEN X,KANG R,KROEMER G,et al.Broadening horizons:The role of ferroptosis in cancer［J］.Nat Rev Clin Oncol,2021,18(5):280-296.
［30］庞敬瑶，李佩玲，Beilerli Aferin，等.基于铁死亡的癌症治疗新策略［J］.现代肿瘤医学，2021，29（6）：1058-1061. PANG JY,LI PL,BEILERLI AFERIN,et al.New strategies of cancer therapy based on ferroptosi［J］.Modern Oncology，2021，29（6）：1058-1061.
［31］ZHANG C,LIU N.Ferroptosis,necroptosis,and pyroptosis in the occurrence and development of ovarian cancer［J］.Front Immunol,2022,13:920059.

Memo

Memo:
National Natural Science Foundation of China（No.81903644,81860458，82260721）;国家自然科学基金青年基金（编号:81903644,81860458，82260721）；广西自然科学基金回国基金（编号:2018GXNSFAA281227，2021GXNSFAA075002）
Last Update: 2024-01-30