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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 04

Page:

601-609

Research Field:

Publishing date:

Info

Title:

Analysis of anoikis-related miRNA spredicting prognosis in hepatocellular carcinoma

Author(s):

LIN Yingxue¹; CUI Haipeng²; YAO Yinhui¹; MENG Xin¹; WANG Ying¹

1.Department of Pharmacy,Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China;2.Department of Pathophysiology,Chengde Medical University,Hebei Chengde 067000,China.

Keywords:

HCC; anoikis; miRNAs; prognosis

PACS:

R735.7

DOI:

10.3969/j.issn.1672-4992.2024.04.003

Abstract:

Objective:Use The Cancer Genome Atlas (TCGA) database to screen anoikis-related miRNAs (armiRNAs) in hepatocellular carcinoma (HCC) tissues and construct a prognostic model based on the selected miRNAs.To explored the relevance of armiRNAs and the prognosis of HCC,hope to provide new targets for liver cancer therapy.Methods:RNA transcription data were downloaded from the TCGA.The differentially expressed armiRNAs were screened in HCC and paraneoplastic tissues.Univariate Cox regression and LASSO regression analyses were used to identify prognostically significant armiRNAs.The reliability was assessed by ROC curves,then a prognostic nomogram model was constructed.Using this model,gene enrichment analysis and drug susceptibility studies were performed using the Kyoto Gene and Genome Encyclopedia (KEGG).Validation of differentially expressed armiRNAs in human normal hepatocyte cell line LO2 and three human hepatocellular carcinoma cell lines by RT-qPCR.Results:This study screened seven armiRNAs,then classified the liver cancer samples into high-risk and low-risk groups according to risk scores.Kaplan-Meier survival curves showed significant differences between the two groups.Mortality increased with risk scores.The AUC of the ROC curves predicting 1-,2- and 3-year survival were 0.794,0.789,and 0.781,indicating a higher degree of discrimination in the model.High-risk groups had higher susceptibility to multiple anti-tumour drugs.Compared with LO2 cells,the expression of hsa-miR-4661,hsa-miR-1180 was significantly increased in three kinds of human HCC cell lines,the expression of hsa-miR-139,hsa-miR-101-2,hsa-miR-326,and hsa-miR-29c was significantly decreased,whereas hsa-miR-509-3-5p was significantly reduced in HepG2 cells and significantly increased in SMMC-7721 and MHCC97 cells.Conclusion:This study identified seven armiRNAs.Models which based on these genes are good predictors of survival and drug sensitivity in patients with HCC.These results may provide some guidance for the exploration of prognostic markers and clinical individualized treatment of HCC.

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Memo

Memo:

河北省医学科学研究课题计划（编号：20200183）；河北省高等学校科学研究青年基金（编号：QN2023017）；河北省中医药类科研计划项目（编号：2022152）

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Last Update: 1900-01-01