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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 04

Page:

610-617

Research Field:

Publishing date:

Info

Title:

Screening of prognosis-related lncRNAs in non-small cell lung cancer and the effect of lncRNA AC099850.3 on cancer cell proliferation,migration,and invasion

Author(s):

MA Wenbo; ZHOU Junliang; XU Hui

Department of Clinical Laboratory,Harbin Medical University Cancer Hospital,Heilongjiang Harbin 150081,China.

Keywords:

non-small cell lung cancer; lncRNA AC099850.3; bioinformatics; proliferation; invasion; metastasis; biological behavior

PACS:

R734.2

DOI:

10.3969/j.issn.1672-4992.2024.04.004

Abstract:

Objective:To screen the abnormally expressed and prognosis-related lncRNAs in non-small cell lung cancer (NSCLC) and to investigate the impact of lncRNA AC099850.3 on NSCLC cell proliferation,migration,and invasion.Methods:Transcriptomic and clinical data from the TCGA database were analyzed to identify lncRNAs associated with NSCLC.LncRNA AC099850.3 was selected based on its high expression,risk value£¾1,significant difference and poor survival outcomes.Expression levels of AC099850.3 were measured in NSCLC cells and tissues using qRT-PCR.AC099850.3 was knocked down in H1299 cell line,cell proliferation was detected by CCK-8 assay,and cell migration was detected by scratch assay.Overexpression of AC099850.3 in A549 cell line,EdU and CCK-8 assay was used to detect the proliferation ability of A549 cells.The migration and invasion ability of A549 cells were detected by Transwell and scratch assay.Results:Through the analysis of TCGA database data,candidate genes AC099850.3,AL365181.2 and NKILA were identified.In patients with lung adenocarcinoma,high expression of AC099850.3 was associated with reduced survival time.The expression of AC099850.3 in cancer tissues of NSCLC patients was significantly higher than that in adjacent tissues.Compared with normal cells,AC099850.3 was highly expressed in A549 and H1299 cells.Knockdown of AC099850.3 significantly reduced the proliferation and migration of H1299 cells.Overexpression of AC099850.3 significantly enhanced the proliferation,invasion and migration of A549 cells.Conclusion:AC099850.3 is upregulated in NSCLC tissues and cell lines.It promotes cell proliferation,migration,and invasion of NSCLC.It has potential as a diagnostic marker and therapeutic target for NSCLC.

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Memo

Memo:

National Natural Science Foundation of China(No.31600662£©£»¹ú¼Ò×ÔÈ»¿Æѧ»ù½ðÇàÄê»ù½ð×ÊÖúÏîÄ¿(±àºÅ£º31600662);ºÚÁú½­Ê¡×ÔÈ»¿Æѧ»ù½ð(±àºÅ£ºLH2023H092)

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