|Table of Contents|

Galangin inhibits proliferation,migration and invasion of thyroid cancer cells by regulating Hippo signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 04
Page:
595-600
Research Field:
Publishing date:

Info

Title:
Galangin inhibits proliferation,migration and invasion of thyroid cancer cells by regulating Hippo signaling pathway
Author(s):
MAO Hongxu; WANG Guangyuan; MENG Xiangning; LI Hongyu
Department of General Surgery,the People's Hospital of Liupanshui City,Guizhou Liupanshui 553001,China.
Keywords:
Galangin; Hippo signaling pathway; thyroid cancer cells; proliferation; migration; invasion
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2024.04.002
Abstract:
Objective:To study the impacts of Galangin on the proliferation,migration and invasion of thyroid cancer cells and the role of Hippo signaling pathway.Methods:Human thyroid cancer cell line 8505C was cultured.CCK-8 assay was used to detect the effect of different concentrations of Galangin on the survival rate of 8505C cells to screen the concentration of Galangin for experiment.8505C cells were divided into control group,Galangin group,Galangin+DMSO group,Galangin+XMU-MP-1 group.EDU method was applied to detect the proliferation of 8505C cells in each group.Transwell was applied to detect 8505C cell invasion in each group.Scratch test was used to detect 8505C cell migration in each group.Flow cytometry was applied to detect the apoptosis rate of 8505C cells in each group.RT-qPCR experiment was applied to detect the mRNA levels of MST1,YAP,and TAZ in 8505C cells in each group.Western blot experiment was applied to detect the protein levels of MST1,YAP,TAZ,MMP2,Ki67,cleaved-Caspase3,Caspase3 in 8505C cells in each group.Results:Compared with the control group,the rate of EDU positive cells,the number of invasive cells,scratch healing rate,the levels of YAP and TAZ mRNAs and proteins,the levels of MMP2,Ki67 proteins of 8505C cells in the Galangin group were obviously decreased,the apoptosis rate,the levels of MST1 mRNA and the levels of MST1,cleaved-Caspase3/Caspase3 protein were obviously increased (P＜0.05).Compared with the Galangin+DMSO group,the rate of EDU positive cells,the number of invasive cells,scratch healing rate,the levels of YAP and TAZ mRNAs and proteins,the levels of MMP2,Ki67 proteins of 8505C cells in the Galangin+XMU-MP-1 group were obviously increased,the apoptosis rate,the levels of MST1 mRNA and the levels of MST1,cleaved-Caspase3/Caspase3 protein were obviously reduced (P＜0.05).Conclusion:Galangin may inhibit the proliferation,migration and invasion of thyroid cancer cell line 8505C by activating Hippo signaling pathway.

References:

［1］TANG X,SHI L,ZHAO Z,et al.Clinical role of 18F-FDG PET/CT for detection of radioactive iodine refractory differentiated thyroid cancer［J］.Medicine (Baltimore),2023,102(24):3878-3886.
［2］FU L,ZHAO L,LIAO C,et al.Knockdown of KAT5/KIF11 induces autophagy and promotes apoptosis in anaplastic thyroid cancer cells［J］.Exp Ther Med,2023,25(6):247-260.
［3］YANG J,BARLETTA JA.Anaplastic thyroid carcinoma［J］.Semin Diagn Pathol,2020,37(5):248-256.
［4］ALOUD AA,CHINNADURAI V,GOVINDASAMY C,et al.Galangin,a dietary flavonoid,ameliorates hyperglycaemia and lipid abnormalities in rats with streptozotocin-induced hyperglycaemia［J］.Pharm Biol,2018,56(1):302-308.
［5］CHIEN ST,SHI MD,LEE YC,et al.Galangin,a novel dietary flavonoid,attenuates metastatic feature via PKC/ERK signaling pathway in TPA-treated liver cancer HepG2 cells［J］.Cancer Cell Int,2015,15(1):15-22.
［6］卢丹,彭小兰,熊珊,等.高良姜素促进胰腺癌PCNA-1细胞凋亡和自噬并抑制移植瘤生长［J］.广州中医药大学学报,2021,38(9):1963-1971. LU D,PENG XL,XIONG S,et al.Galangin promotes apoptosis and autophagy in pancreatic cancer PCNA-1 cells and inhibits xenograft growth［J］.Journal of Guangzhou University of Traditional Chinese Medicine,2021,38(9):1963-1971.
［7］SALEM O,HANSEN CG.The Hippo pathway in prostate cancer［J］.Cells,2019,8(4):370-382.
［8］MOYA IM,HALDER G.Hippo-YAP/TAZ signalling in organ regeneration and regenerative medicine［J］.Nat Rev Mol Cell Biol,2019,20(4):211-226.
［9］LIU X,FU Q,BIAN X,et al.Long non-coding RNA MAPK8IP1P2 inhibits lymphatic metastasis of thyroid cancer by activating Hippo signaling via sponging miR-146b-3p［J］.Front Oncol,2021,10(1):27-36.
［10］杨寒,任珊,刘茂伦,等.基于Hippo信号通路的野黄芩苷抗结直肠癌HCT116细胞的研究［J］.天然产物研究与开发,2023,35(1):22-32. YANG H,REN S,LIU ML,et al.Suppression of colorectal cancer HCT116 cells by scutellarin viaregulation of Hippo signaling pathway［J］.Natural Product Research and Development,2023,35(1):22-32.
［11］陈淑梅.高良姜素诱导人乳头瘤病毒阳性的宫颈癌细胞凋亡实验研究［J］.中草药,2017,48(5):941-945. CHEN SM.Inductive effects of galangin on apotosis of human papilloma virus positive cervical cancer cells［J］.Chinese Traditional and Herbal Drugs,2017,48(5):941-945.
［12］陈明明,李克寒,刘相乐,等.依托咪酯通过调节Hippo信号通路抑制鼻咽癌细胞CNE-1和HNE-1的侵袭迁移［J］.广东药科大学学报,2020,36(6):840-846. CHEN MM,LI KH,LIU XL,et al.Etomidate inhibits invasion and migration of nasopharyngeal carcinoma cells CNE-1 and HNE-1 by regulating Hippo signaling pathway［J］.Journal of Guangdong Pharmaceutical University,2020,36(6):840-846.
［13］PRETE A,BORGES DE SOUZA P,CENSI S,et al.Update on fundamental mechanisms of thyroid cancer［J］.Front Endocrinol (Lausanne),2020,11(1):102-113.
［14］TEIXEIRA MP,HADDAD NF,PASSOS EF,et al.Ouabain effects on human anaplastic thyroid carcinoma 8505C cells［J］.Cancers (Basel),2022,14(24):6168-6180.
［15］KOPUSTINSKIENE DM,JAKSTAS V,SAVICKAS A,et al.Flavonoids as anticancer agents［J］.Nutrients,2020,12(2):457-468.
［16］RODRGUEZ-GARCA C,SNCHEZ-QUESADA C,J GAFORIO J.Dietary flavonoids as cancer chemopreventive agents:an updated review of human studies［J］.Antioxidants (Basel),2019,8(5):137-147.
［17］SONG W,YAN CY,ZHOU QQ,et al.Galangin potentiates human breast cancer to apoptosis induced by TRAIL through activating AMPK［J］.Biomed Pharmacother,2017,89(1):845-856.
［18］LONG X,CHEN L,YANG J,et al.Network-based pharmacology and in vitro validation reveal that galangin induces apoptosis in bladder cancer cells by promoting the P53 signaling pathway［J］.Anticancer Agents Med Chem,2023,23(7):847-857.
［19］WANG MZ,ZHOU Y,HE KT,et al.Research progress on the mechanism of Hippo pathway in tumorigenesis and development［J］.Modern Oncology,2022,30（16）：3061-3064.
［20］CALSES PC,CRAWFORD JJ,LILL JR,et al.Hippo pathway in cancer:aberrant regulation and therapeutic opportunities［J］.Trends Cancer,2019,5(5):297-307.
［21］MOHAJAN S,JAISWAL PK,VATANMAKARIAN M,et al.Hippo pathway:regulation,deregulation and potential therapeutic targets in cancer［J］.Cancer Lett,2021,507(1):112-123.

Memo

Memo:
贵州省六盘水市科技计划项目(编号：52020-2020-0-2-10)
Last Update: 1900-01-01