|Table of Contents|

Inhibition of prostate cancer cell proliferation and invasion by agrimonolide via the Wnt/β-catenin signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 04
Page:
589-595
Research Field:
Publishing date:

Info

Title:
Inhibition of prostate cancer cell proliferation and invasion by agrimonolide via the Wnt/β-catenin signaling pathway
Author(s):
NIE WeidongJIA MoranSHAO YiqunSHENG DongyaPENG Yu
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China.
Keywords:
agrimonolideprostate cancerproliferationapoptosisinvasionWnt/β-catenin
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2024.04.001
Abstract:
Objective:To investigate the effects of agrimonolide (AM) on proliferation and invasion of prostate cancer cells,and its mechanism of action.Methods:The cell counting kit-8 (CCK8) assay and colony formation assay were used to assess the influence of AM on cell proliferation.Flow cytometry was employed to examine the impact of AM on apoptosis of prostate cancer cells.The Transwell assay was conducted to evaluate the effect of AM on cell invasion.Western blot analysis was employed to investigate the effects of AM on proteins related to the Wnt/β-catenin signaling pathway in prostate cancer cells.Additionally,the DU145 cell line overexpressing β-catenin was established using lentiviral infection technology.Results:Compared with the control group,AM treatment led to a gradual decrease in cell viability of prostate cancer cells,with respective IC50 of values 20.33 μmol/L,21.05 μmol/L.AM significantly inhibited colony formation of prostate cancer cells in a concentration-dependent manner.AM treatment resulted in a significant increase in apoptosis rate of prostate cancer cells compared with the control group,showing a concentration-dependent effect.AM treatment markedly suppressed the expression of Wnt/β-catenin signaling pathway related protein β-catenin,p-GSK3β/GSK3β,c-Myc and Cyclin D1 in prostate cancer cells compared with the control group.In the DU145 cell line overexpressing β-catenin,the inhibitory effects of AM on tumor proliferation and metastasis were significantly attenuated.Conclusion:AM can inhibit the proliferation and invasion of prostate cancer cells by inducing cell apoptosis through the suppression of the Wnt/β-catenin signaling pathway.

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Memo

Memo:
National Natural Science Foundation of China(No.82104720);国家自然科学基金项目(编号:82104720)
Last Update: 1900-01-01