|Table of Contents|

Silencing lncRNA MCF2L-AS1 inhibits proliferation,migration and invasion of breast cancer cells via miR-138-5p/AnxA2 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 02
Page:
240-248
Research Field:
Publishing date:

Info

Title:
Silencing lncRNA MCF2L-AS1 inhibits proliferation,migration and invasion of breast cancer cells via miR-138-5p/AnxA2 axis
Author(s):
WANG Jinli; CHEN Dengfeng
Breast Department,Jingzhou Central Hospital,Hubei Jingzhou 434020,China.
Keywords:
lncRNA MCF2L-AS1; miR-138-5p/AnxA2 axis; breast cancer; proliferation; migration; invasion
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.02.007
Abstract:
Objective:To explore the effects and mechanisms of lncRNA MCF2L-AS1 on proliferation,migration and invasion of breast cancer cells.Methods:The levels of lncRNA MCF2L-AS1 and miR-138-5p in normal human breast epithelial cell line MCF-10A and breast cancer cell lines (MDA-MB-415,MCF7,SKBR3,MDA-MB-231) were detected by qRT-PCR.si-NC,si-MCF2L-AS1,si-MCF2L-AS1+inhibitor NC,and si-MCF2L-AS1+miR-138-5p inhibitor were transfected into MDA-MB-415 cells respectively,and named as si-NC group,si-MCF2L-AS1 group,si-MCF2L-AS1+inhibitor NC group,and si-MCF2L-AS1+miR-138-5p inhibitor group.MDA-MB-415 cells without any treatment were recorded as NC group.qRT-PCR was applied to detect the expression of lncRNA MCF2L-AS1 and miR-138-5p in MDA-MB-415 cells.MTT method was applied to detect the proliferation of MDA-MB-415 cells.Flow cytometry was applied to detect the apoptosis rate of MDA-MB-415 cells.Transwell was applied to detect the invasion and migration of MDA-MB-415 cells.Western blot was applied to detect the levels of E-cadherin,Vimentin,N-cadherin,and AnxA2 proteins in MDA-MB-415 cells.Double luciferase reporter gene experiment was applied to verify the relationship between lncRNA MCF2L-AS1,miR-138-5p and AnxA2.Mouse xenograft experiment was applied to detect tumor growth.Results:Compared with MCF-10A cells,the levels of lncRNA MCF2L-AS1 and AnxA2 in MDA-MB-415,MCF7,SKBR3 and MDA-MB-231 cells were obviously up-regulated (P＜0.05),while the expression of miR-138-5p was obviously down-regulated (P＜0.05).Compared with the NC group and si-NC group,the OD560 nm value,migration and invasion cells numbers,lncRNA MCF2L-AS1 expression,N-cadherin and Vimentin protein levels,mouse tumor volume,and tumor quality of MDA-MB-415 cells in the si-MCF2L-AS1 group obviously decreased (P＜0.05),the apoptosis rate,miR-138-5p expression,and E-cadherin protein level of MDA-MB-415 cells were obviously increased (P＜0.05).The inhibiting the expression of miR-138-5p weakened the inhibitory effect of silencing lncRNA MCF2L-AS1 on the proliferation,migration,invasion,and tumor growth of MDA-MB-415 cells.lncRNA MCF2L-AS1 negatively regulated the miR-138-5p/AnxA2 axis.Conclusion:Silencing lncRNA MCF2L-AS1 may down regulate the expression of AnxA2 by up-regulating miR-138-5p,thereby inhibiting the proliferation,migration and invasion of breast cancer MDA-MB-415 cells.

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荆州市医疗卫生科技计划项目（指导性计划）（编号：2021HC10）
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