|Table of Contents|

Garcinol exerts the cancer-inhibiting role in cervical cancer through regulation of PTEN

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 02
Page:
234-240
Research Field:
Publishing date:

Info

Title:
Garcinol exerts the cancer-inhibiting role in cervical cancer through regulation of PTEN
Author(s):
FENG Sifang1; ZHAO Juan2; YANG Ting2; LI Chen2; LI Long2
1.Department of Respiratory and Critical Care Medicine;2.Department of Obstetrics and Gynecology,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.
Keywords:
cervical cancer; garcinol; PTEN; CRISPR/Cas9
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.02.006
Abstract:
Objective:To investigate whether garcinol exerts the cancer-inhibiting role in cervical cancer through regulation of PTEN.Methods:PTEN expression was measured by immunoblotting.PTEN-knockout (KO) cervical cancer cells were established using CRISPR/Cas9 technology.Cell growth and proliferation were detected by CCK-8 assay and EdU assay.Cell apoptosis was assessed by flow cytometry.Cell migration was measured by wound-healing assay.Cell invasion was evaluated by Transwell assay.Xenograft tumors in nude mice derived from PTEN-KO cells were applied to assess the effect of garcinol on tumor growth.PTEN expression and Ki-67 proliferative index in xenograft tumors were determined by immunohistochemical staining.Results:Treatment with garcinol significantly upregulated the expression of PTEN protein in cervical cancer cells (P＜0.01).PTEN was markedly knocked out in cells transfected with Cas9-PTEN vectors (P＜0.01).In wild-type cervical cancer cells,garcinol was able to inhibit cell proliferation,migration and invasion,and induce cell apoptosis (P＜0.01).In PTEN-KO cervical cancer cells,garcinol showed no obvious effect on cell proliferation,migration,invasion and apoptosis (P＞0.05).PTEN-KO blocked the suppressive effect of garcinol on the growth of xenograft tumors in nude mice.Conclusion:Garcinol exerts the cancer-inhibiting role in cervical cancer through regulation of PTEN.

References:

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西安交通大学第一附属医院科研发展基金(编号：2021ZXY-16)
Last Update: 1900-01-01