|Table of Contents|

Effects of MEX3A on malignant phenotype of colon cancer cells and its mechanism of action

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 02
Page:
226-233
Research Field:
Publishing date:

Info

Title:
Effects of MEX3A on malignant phenotype of colon cancer cells and its mechanism of action
Author(s):
ZHOU Junyi1; SHA Xiaofeng2; LIU Ye1; HAN Zhuoying3; DING Shu3; HUANG Hao1; LUO Chao3; ZHONG Xiaomin1
1.The Huai'an Clinical College of Xuzhou Medical University,Jiangsu Huai'an 223300,China;2.Department of Oncology,Hongze District People's Hospital,Jiangsu Huai'an 223100,China;3.Central Laboratory of Department of Oncology,the Affiliated Huai'an No.1 Hospital of Nanjing Medical University,Jiangsu Huai'an 223300,China.
Keywords:
MEX3A; colon cancer; prognosis; malignant phenotype
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2024.02.005
Abstract:
Objective:To investigate the expression of MEX3A in colon cancer tissue and its effect on the malignant phenotype of colon cancer cells and explore its mechanism.Methods:The TCGA database analyzed the differential expression of MEX3A in colon cancer tissues and normal tissues and its correlation with the clinicopathological stage and prognosis of patients,and the results were further verified by immunohistochemistry.Lentiviral was used to knock down the expression of MEX3A in colon cancer cells to detect the proliferation,apoptosis,migration and invasion ability of cells.Tumor growth was measured and recorded after subcutaneous tumors in nude mice.COIP experiments were performed after Flag fusion expression plasmid overexpression MEX3A.Qualitative detection of proteins was performed using mass spectrometry (Shotgun) interacting with MEX3A.After bioinformatics analysis,COIP verification was performed,and rescue experiments were performed to verify the expression of interactive proteins.Results:The expression of MEX3A in colon cancer tissue was significantly upregulated and correlated with the clinicopathological features and prognosis.After lentiviral knockdown MEX3A expression,the proliferation ability of colon cancer cells was significantly inhibited,and apoptosis was significantly increased.The results of cell metastasis ability test showed that the migration and invasion ability of colon cancer cells were significantly inhibited.The results of in vivo experiments in mice showed that the growth of tumors in vivo was significantly inhibited after knocking out MEX3A expression.Qualitative protein detection and COIP verification results showed that EIF2AK2,LAMB3 and MSI2 interacted with MEX3A proteins.After the expression of knock-down MEX3A,the cell proliferation ability was restored to different degrees after overexpression of EIF2AK2,LAMB3 and MSI2,among which the cell proliferation ability was the most obvious after MSI2 overexpression.The results of MTT and cell migration ability verification showed that after overexpression of MSI2,cell proliferation and migration ability were significantly restored.Conclusion:The expression of MEX3A in colon cancer tissue is upregulated and the prognosis of patients is negatively correlated with MEX3A high expression,and it promotes the malignant phenotype of colon cancer cells by interacting with MSI2.

References:

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江苏省研究生科研与实践创新计划项目（编号：SJCX21_1154，SJCX23_1408）；江苏省双创博士（编号：202031099）；江苏省淮安市创新能力建设-淮安市免疫学重点实验室（编号：HAP202002）；南京医科大学苏北临床研究院转化医学创新人才项目（编号：YZHR201901，YZHR201904）
Last Update: 1900-01-01