|Table of Contents|

Cichoric acid inhibits lung cancer cell proliferation and chemotherapy sensitivity and promotes apoptosis through the FOXO3-FOXM1 signal axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 02
Page:
248-254
Research Field:
Publishing date:

Info

Title:
Cichoric acid inhibits lung cancer cell proliferation and chemotherapy sensitivity and promotes apoptosis through the FOXO3-FOXM1 signal axis
Author(s):
LIU JieLI XinjianJIANG PanJIAO Yuzhi
School of Food,Jiangsu Food and Drug Vocational and Technical College,Jiangsu Huai'an 223001,China.
Keywords:
cichoric acidFOXO3-FOXM1lung cancerproliferationapoptosischemotherapy sensitivity
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.02.008
Abstract:
Objective:To investigate the impacts of cichoric acid on proliferation,apoptosis,and chemotherapy sensitivity of lung cancer cells by regulating the forkhead box O3 (FOXO3) - forkhead protein M1 (FOXM1) signal axis.Methods:Cisplatin (DDP) resistant human lung cancer cell line A549/DDP was constructed and treated with different concentrations of cichoric acid.Cell viability was determined by MTT assay.The maximum concentration that did not significantly reduce cell viability was used as the optimal concentration of cichoric acid.A549 cells were randomly separated into a control group,an empty group,a cichoric acid group,and a cichoric acid+FOXO3 knockdown group.After grouping,MTT assay,colony formation assay,and flow cytometry were applied to determine the proliferation and apoptosis of A549 cells in each group.Immunoblotting was applied to detect the expression of FOXO3-FOXM1 pathway,proliferation (PCNA),and apoptosis (Bcl-2,Bax) related proteins in A549 cells in each group.A549/DDP cells were randomly separated into control group,DDP group,DDP+empty group,DDP+cichoric acid group,and DDP+cichoric acid+FOXO3 knockdown group.After grouping,MTT assay,colony formation assay,and flow cytometry were applied to determine the proliferation and apoptosis of A549/DDP cells in each group.Immunoblotting was applied to detect the expression of FOXO3-FOXM1 pathway,proliferation,resistance[P-glycoprotein (P-gp)],and apoptosis related proteins in A549/DDP cells in each group.Results:Compared with the control group,there was no obvious change in all indicators of A549 cells in the empty group (P>0.05).The apoptosis rate of A549 cells and the expression of Bax and FOXO3 proteins in the cichoric acid group increased (P<0.05),the colony formation rate,cell viability,and the expression of Bcl-2,PCNA,FOXM1 proteins reduced (P<0.05).Knocking down FOXO3 can weaken the effect of cichoric acid on the above indicators in A549 cells.Compared with the control group and DDP group,there was no obvious change in various indicators of A549/DDP cells in the DDP+empty group (P>0.05).The apoptosis rate of A549/DDP cells and the expression of Bax and FOXO3 proteins in the DDP+cichoric acid group increased (P<0.05),the colony formation rate,cell viability,and the expression of Bcl-2,PCNA,P-gp,FOXM1 proteins reduced (P<0.05).Knocking down FOXO3 can weaken the effect of cichoric acid on the above indicators of A549/DDP cells under DDP treatment.Conclusion:Cichoric acid can inhibit lung cancer cell proliferation,chemotherapy sensitivity,and promote apoptosis by activating FOXO3-FOXM1 signaling,thereby enhancing the killing effect of chemotherapy drug DDP on DDP resistant cells.

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江苏省高等学校自然科学研究重大项目(编号:21KJA350001)
Last Update: 1900-01-01