|Table of Contents|

S100P gene silencing inhibits proliferation,migration and invasion of lung adenocarcinoma A549 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
1002-1008
Research Field:
Publishing date:

Info

Title:
S100P gene silencing inhibits proliferation,migration and invasion of lung adenocarcinoma A549 cells
Author(s):
CHENG Anqi1; WU Jing1; 2; 3; LIU Yafeng1; ZHOU Jiawei1; WANG Qingsen1; DONG Yunjia1; HU Dong1; 2; 3
1.School of Medicine,Anhui University of Science and Technology,Anhui Huainan 232000,China;2.Anhui Province Engineering Laboratory of Occupational Health and Safety,Anhui University of Science and Technology,Anhui Huainan 232000,China;3.Key Laboratory of Industrial Dust Prevention and Control & Occupational Safety and Health of the Ministry of Education,Anhui University of Science and Technology,Anhui Huainan 232000,China.
Keywords:
lung cancer; S100P; lentivirus; proliferation; cell cycle; migration
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.06.004
Abstract:
Objective：To investigate the effect of S100P gene expression on proliferation,migration and invasion in lung cancer tissues and cells and its mechanism.Methods:The data of lung cancer were obtained from GEPIA (Gene Expression Profiling Interactive Analysis) database and HPA (Human Protein Atlas) database,and the S100P gene was analyzed for the differential expression of S100P gene in different types of lung cancer tissues and normal tissues.The expression of S100P gene in lung cancer cell line was down regulated by shRNA-S100P RNA.The cell proliferation activity was detected by MTS colorimetry.Cell cycle changes were detected by flow cytometry.Cell scratch test and Transwell test were used to detect the migration ability and invasion ability of cells respectively.RT-qPCR and Western blot were used to verify S100P expression,cell cycle and apoptotic protein expression.Results:The expression level of S100P gene in lung cancer tissues was significantly higher than that in normal tissues(P＜0.01).S100P gene expression was different in different types of lung cancer.Compared with the control group,down-regulation of S100P reduced the proliferation,migration and invasion of A549.Conclusion:S100P gene overexpression and down-regulation in lung cancer can inhibit the proliferation,migration and invasion of A549 cells.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.81971483,81672445);国家自然科学基金(编号：81971483,81672445）;安徽省高校协同创新项目（编号：GXXT-2020-058）
Last Update: 1900-01-01