|Table of Contents|

Effect of immune related gene HCST on prognosis of clear cell renal cell carcinoma and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
997-1001
Research Field:
Publishing date:

Info

Title:
Effect of immune related gene HCST on prognosis of clear cell renal cell carcinoma and its mechanism
Author(s):
LYU Lei; ZHENG Fuxin; WU Wei; ZHOU Gaofeng
Department of Urology,Wuhan No.1 Hospital,Hubei Wuhan 430022，China.
Keywords:
hematopoietic cell signal transducer; clear cell renal cell carcinoma; immune infiltration; prognosis
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2023.06.003
Abstract:
Objective：To identify the expression of hematopoietic cell signal transducer (HCST) in clear cell renal cell carcinoma (ccRCC) and its relationship with the prognosis and immune infiltration of ccRCC,and to explore the effects of HCST on cell growth and invasion in ccRCC and its mechanism.Methods:HCST expression level in ccRCC tissues was analyzed by using TCGA database.The relationships between HCST expression level and clinical features and prognosis of ccRCC patients were determined by Cox proportional-hazards model.Tumor immune estimation resource (TIMER) was utilized to analyze the correlation between the expression level of HCST and immune cell infiltration in ccRCC.ACHN cells were cultured in vitro and divided into three groups：Control group,negative control siRNA (NC siRNA) group and HCST siRNA group.After transfection,MTT assay,flow cytometry assay and transwell assay were performed to detect the proliferation,apoptosis and invasiveness of ACHN cells,respectively.Western blot was used to determine the protein levels of caspase-3,Bax,Bcl-2,E-cadherin,N-cadherin and Vimentin.Results：Compared with normal kidney tissues,the expression of HCST mRNA was significantly up-regulated in ccRCC tissues,and showed a positive correlation with tumor stage,distant metastasis and lymph node metastasis (P＜0.01).High expression of HCST,pathological T stage and M stage were independent risk factors for the prognosis of ccRCC patients (P＜0.05).TIMER database analysis revealed a positive correlation between HCST expression and infiltration degree of CD8+ T cells,dendritic cells,NK cells and Treg cells (P＜0.001).Compared with control group,the cell viability,invasiveness,Bcl-2,N-cadherin and Vimentin protein expression levels were significantly decreased in HCST siRNA group (P＜0.05),and the apoptosis activity,caspase-3,Bax and E-cadherin were significantly increased (P＜0.05).There was no significant difference in the above indicators between the control group and NC siRNA group (P＞0.05).Conclusion：In ccRCC,HCST is highly expressed and is associated with immune infiltration and a poor prognosis.Silencing HCST could inhibit the proliferation and invasion and promote apoptosis of ccRCC cells.

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Memo

Memo:
National Natural Science Foundation of China(No.81902593);国家自然科学基金(编号：81902593)；湖北省自然科学基金(编号：2020CFB732)；湖北省武汉市科技计划项目(编号：2020020601012325)
Last Update: 1900-01-01