|Table of Contents|

Silencing circ9119 inhibits proliferation,invasion and autophagy of hepatocellular carcinoma cells by regulating miR-126

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
1008-1014
Research Field:
Publishing date:

Info

Title:
Silencing circ9119 inhibits proliferation,invasion and autophagy of hepatocellular carcinoma cells by regulating miR-126
Author(s):
REN Na1; HU Shanlei2; LIU Jinjin3; LI Guangming2; MA Chao4
1.Department of General Medicine,Henan Provincial Hospital,Henan Zhengzhou 450000，China;2.Department of Liver Cirrhosis;3.Translational Medicine Center,the Sixth People's Hospital of Zhengzhou,Henan Zhengzhou 450000，China;4.Department of Hepatobiliary and Pancreatic Surgery,Affiliated Cancer Hospital of Zhengzhou University,Henan Zhengzhou 450000,China.
Keywords:
circ9119; miR-126; hepatocellular carcinoma; proliferation; invasion; autophagy; apoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.06.005
Abstract:
Objective：To identify the role and mechanism of circular-RNA-9119 (circ9119) in hepatocellular carcinoma (HCC).Methods:The expression of circ9119 and miR-126 in the collected tissue samples and purchased cell lines were detected by qRT-PCR,and cells were screened.FISH assay,RIP assay and luciferase reporter assay were peroformed to confirm the relationship between circ9119 and miR-126.Apoptosis related factors and autophagy markers were tested by qRT-PCR and Western blot.The proliferation,invasion and apoptosis of HCC cells were measured by CCK8 assay,Transwell assay and flow cytometry assay respectively.Tumor growth was observed in nude mice.Results:The expression of circ9119 was increased in HCC tissue and cells,while the expression of miR-126 was decreased in HCC tissues and cell (P＜0.05).circ9119 could regulate miR-126 by functioning as a ceRNA.Silencing of miR-126 could inhibit the proliferation,invasion and autophagy,promote the apotposis of HCC cells,and restrain the growth of tumor in mice (P＜0.05).However,the effects of overexpression of circ9119 was opposite to that.The changes in cells of up-regulating miR-126 expression and down-regulating circ9119 expression were in the same trend.The effects of up-regulating miR-126 expression could be reversed by overexpression of circ9119.Conclusion:Together,our findings suggested that circ9119 functions as an oncogene in HCC by regulating miR-126 negatively.

References:

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