|Table of Contents|

Bioinformatics analysis of differentially expressed genes in castration-resistant prostate cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
481-487
Research Field:
Publishing date:

Info

Title:
Bioinformatics analysis of differentially expressed genes in castration-resistant prostate cancer
Author(s):
WANG Jinhong; SHEN Gang; Huang Zhijian; Shi Jinhui; YANG Xia; GAO Guoquan; ZHOU Ti
Sun Yat-sen University Zhongshan School of Medicine,Guangdong Guangzhou 510080,China.
Keywords:
castration-resistant prostate cancer; primary prostate cancer; bioinformatics analysis; GEO; differential gene
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2022.03.021
Abstract:
Objective:To screen the differentially expressed genes(DEGs) between primary prostate cancer(PCa) and castration-resistant prostate cancer(CRPC) at the molecular level via big data.The aim is to explore the possible mechanism of the development of prostate cancer cells from androgen dependence to resistance,which provides a target for the diagnosis and treatment of CRPC.Methods:Three gene expression chips data of CRPC(GSE74367,GSE66187 and GSE126881) were downloaded from GEO datasets.The limma package was used for the standardization and DEGs analysis.The clusterProfiler package was used for GO function and KEGG pathway analysis.Using P＜0.05 and |logFC|>2 as the screening criteria,the vennDiagram package was used to merge gene sets to find common DEGs of the three chips.And the clusterProfiler package was further used to analyze the common up-regulated or down-regulated genes by GSEA.Results:The DEGs of GSE74367 and GSE66187 were mainly related to RNA catabolism and splicing,and they were mainly enriched in the ribosomal biogenesis pathway.The DEGs in GSE126881 were mainly expressed in chemical carcinogenesis,steroid hormone biosynthesis and the metabolism of retinol,xenobiotics of cytochrome P450,ascorbate and aldarate.SlC16A3 was the common up-regulated gene,and DDX53 was the common down-regulated gene in these 3 chips.The GSEA showed that when SLC16A3 was up-regulated,fructose,mannose,and pentose phosphate metabolism and cell cycle-related gene sets were significantly up-regulated.When DDX53 was down-regulated,the gene sets related to primary immunodeficiency,chemokines,and cell adhesion molecules were down-regulated,while the gene sets related to pentose phosphate pathway,cell cycle,and protein export were up-regulated.Conclusion:Through bioinformatics analysis of three CRPC related GEO chips,we found that the up-regulation of SLC16A3 and down-regulation of DDX53 may play an important role in the development of PCa into CRPC.

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Memo

Memo:
National Natural Science Foundation of China(No.81172163）；国家自然科学基金（编号：81172163）
Last Update: 2021-12-31