|Table of Contents|

Thioredoxin interacting protein promotes the occurrence of colon adenocarcinoma cell apoptosis by inhibiting autophagy pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
395-401
Research Field:
Publishing date:

Info

Title:
Thioredoxin interacting protein promotes the occurrence of colon adenocarcinoma cell apoptosis by inhibiting autophagy pathway
Author(s):
ZHANG Lifang1; YANG Jielin2; HU Xiaoli2; XU Jiang1
1.Department of Internal Medicine,Qinhuangdao Orthopedic Hospital,Hebei Qinhuangdao 066001,China;2.Department of Gastroenterology,the First Affiliated Hospital of Hebei North University,Hebei Zhangjiakou 075000,China.
Keywords:
colon adenocarcinoma; thioredoxin interacting protein; apoptosis; autophagy
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2022.03.006
Abstract:
Objective:To explore the effect and mechanism of overexpression of thioredoxin interacting protein(TXNIP) on the apoptosis of colon adenocarcinoma cells.Methods:Collect colon adenocarcinoma and paracancerous tissues from 30 patients with colon adenocarcinoma in our hospital.Real-time fluorescence quantitative PCR detected the expression of TXNIP mRNA in the tissue.Immunohistochemical staining was used to detect the expression of autophagy marker protein LC3-Ⅱ and apoptosis marker protein Cleaved Caspase-3 in tissues.Human colon adenocarcinoma LoVo cells were randomly divided into control group,negative empty vector group(LV-GFP) and TXNIP overexpression group(LV-GFP-TXNIP).Observe cell transfection efficiency under an inverted fluorescence microscope.Collect LoVo cells 72 h after transfection,detect cell apoptosis by flow cytometry.Transmission electron microscope observes the autophagy of cells.Immunofluorescence staining detected the expression of LC3 in cells.Western blot was used to detect changes in the expression of autophagy-related proteins and apoptosis-related proteins in cells.Results:Compared with adjacent tissues,the relative expression of TXNIP mRNA in the colon adenocarcinoma tumor tissue was significantly decreased(P＜0.01),the positive expression rate of LC3-Ⅱ was significantly increased(P＜0.05),and the positive expression rate of Cleaved Caspase-3 significantly reduced(P＜0.05).72 h after lentivirus transfection,the transfection efficiency was higher(P＜0.05).Compared with the control group,the apoptosis rate of LoVo cells in the LV-GFP-TXNIP group was significantly increased(P＜0.05),the cytoplasm was denser,the number of autophagosomes was significantly reduced,the fluorescence staining of LC3 was weakened,and the expression of Beclin-1 and LC3-Ⅱ proteins significantly decreased(P＜0.05),while the expression of LC3-I and p62 protein increased significantly(P＜0.05).At the same time,the protein expression of Bax and Cleaved Caspase-3 increased significantly(P＜0.05),and the protein expression of Bcl-2 decreased significantly(P＜0.05).Conclusion:Overexpression of TXNIP in colon adenocarcinoma cells can promote apoptosis by inhibiting autophagy.

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Memo

Memo:
河北省卫健委青年科技课题（编号：20190874）
Last Update: 2021-12-31