|Table of Contents|

Silencing EMI2 gene inhibits proliferation and epithelied-mesenchymal transformation of intrahepatic cholangiocarcinoma HuCCT1 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
389-395
Research Field:
Publishing date:

Info

Title:
Silencing EMI2 gene inhibits proliferation and epithelied-mesenchymal transformation of intrahepatic cholangiocarcinoma HuCCT1 cells
Author(s):
ZHOU Shuai1; QU Kanglin1; SU Lili2; CHEN Shilei1; LI Jinang1; JIN Hao3; WANG Yong3; PANG Qing1; LIU Huichun1
1.Department of Hepatobiliary Surgery;2.Department of Oncology,the First Affiliated Hospital of Bengbu Medical College,Anhui Bengbu 233000,China;3.The Fourth Department of General Surgery,the Second People's Hospital of Anhui Province,Anhui Hefei 230000,China.
Keywords:
early mitotic suppressor 2; intrahepatic cholangiocarcinoma; proliferation; epithelial-mesenchymal transformation; invasion; migration
PACS:
R735.8
DOI:
10.3969/j.issn.1672-4992.2022.03.005
Abstract:
Objective:To explore the expression and biological function of early mitotic inhibitor 2(EMI2) in intrahepatic cholangiocarcinoma(iCCA) tissues.To investigate the effects of EMI2 on the proliferation,invasion and epithelial-mesenchymal transformation of iCCA cells HuCCT1.Methods:Bioinformatics technique was used to detect the expression of EMI2 in bile duct carcinoma.Gene silencing technique was used to silence EMI2,and its effects on proliferation,invasion,migration and EMT of HuCCT1 cells were observed.Results:Bioinformatics showed that EMI2 was highly expressed in cholangiocarcinoma tissues,while Western Blot and RT-PCR were ineffective in detecting EMI2.CCK-8 showed that the OD450 value of shEMI2 group was lower than that of shCtrol group(P＜0.05),and the number of shEMI2 cell clone formation(26±2) was lower than that of shCtrol group(57±4).Invasive results showed that the number of invasion in shEMI2 group was (39±4.92) and in shCtrol group was (195±14.00).Cell cycle test showed that compared with shCtrol group,shEMI2 group of G2/M cells decreased and G1 cells increased(P＜0.05).The apoptosis experiment showed that the apoptosis rate of shEMI2 group was increased(P＜0.05).Western Blot and immunofluorescence results suggested that silencing EMI2 could promote the up-regulation of E-cadherin protein and down-regulation of N-cadherin and Vitmentin protein.Conclusion:Silencing EMI2 gene inhibites the proliferation,invasion,migration and epithelied-mesenchymal transformation of intrahepatic cholangiocarcinoma.

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Memo

Memo:
安徽省自然科学基金杰出青年基金(编号：2008085J37）;蚌埠医学院研究生科研创新计划基金（编号：Byycx20032）
Last Update: 2021-12-31