|Table of Contents|

The expression of Glypican-6 in gastrointestinal stromal tumor tissues and it promotes the proliferation,migration and invasion of cells through the ERK1/2 pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
382-388
Research Field:
Publishing date:

Info

Title:
The expression of Glypican-6 in gastrointestinal stromal tumor tissues and it promotes the proliferation,migration and invasion of cells through the ERK1/2 pathway
Author(s):
GONG Qinghao; GAO Pingfa; GU Zhixiang; CAI Yiting
Department of Gastrointestinal Surgery,Chongming Branch of Shanghai Xinhua Hospital,Shanghai 202150，China.
Keywords:
Glypican-6; gastrointestinal stromal tumor; ERK1/2 pathway
PACS:
R735
DOI:
10.3969/j.issn.1672-4992.2022.03.004
Abstract:
Objective:To explore the effect of Glypican-6 on gastrointestinal stromal tumors and its possible mechanism.Methods:Twenty-seven cases of gastrointestinal stromal tumor tissues and adjacent tissues were collected,and the expression of Glypican-6 in the tissues was detected by RT-PCR and Western blot.The gastrointestinal stromal tumor cell line GIST-T1 was selected for vitro studies.RT-PCR experiment was used to detect cell Glypican-6 mRNA expression.Western blot was used to detect cell Glypican-6,E-cadherin,N-cadherin,p-ERK1/2 and ERK1/2 protein expression.CCK-8 was used to detect cell proliferation.Transwell test was used to detect cell migration and invasion.Results:Compared with adjacent tissues,mRNA and protein expression of Glypican-6 in cancer tissues were significantly increased(P＜0.05).Compared with the si-NC group,the expression of Glypican-6 mRNA and protein in si-Glypican-6 cells were significantly reduced(P＜0.05).The proliferation capacity of cells at 24 h,48 h and 72 h,number of migrating cells and number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly reduced(P＜0.05),and the expression of E-cadherin protein was significantly increased(P＜0.05).Compared with the pcDNA3.1 group,the expression of Glypican-6 mRNA and protein in the cells of pcDNA3.1- Glypican-6 group were significantly increased(P＜0.05),and the proliferation ability at 24 h,48 h and 72 h,number of migrating cells,number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly increased(P＜0.05),and the expression of E-cadherin protein was significantly decreased(P＜0.05).Compared with the pcDNA3.1-Glypican-6 group,the pcDNA3.1-Glypican-6+PD98059 group had significantly lower proliferation ability at 24 h,48 h and 72 h(P＜0.05),and the number of migrating cells,the number of invading cells and the protein expression of N-cadherin and p-ERK1/2/ERK1/2 rate were significantly reduced(P＜0.05),protein expression of E-cadherin was significantly increased(P＜0.05),and protein expression of Glypican-6 was not significantly different(P>0.05).Conclusion:Glypican-6 is highly expressed in gastrointestinal stromal tumors.Glypican-6 promotes the proliferation,migration,invasion and protein expression of N-cadherin of gastrointestinal stromal tumor cells through ERK1/2 pathway,and inhibits the expression of E-cadherin protein.

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上海市崇明区“可持续发展科技创新行动计划”项目（编号：CKY2020-13）
Last Update: 2021-12-31