|Table of Contents|

lncRNA-NEAT1 inhibits the growth of renal cancer cells by targeting miR-34a

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
376-382
Research Field:
Publishing date:

Info

Title:
lncRNA-NEAT1 inhibits the growth of renal cancer cells by targeting miR-34a
Author(s):
ZHONG Xiao; HUANG Long; ZHU Shuxia
Department of Urology,Chengdu 363 Hospital Affiliated to Southwest Medical University,Sichuan Chengdu 610000,China.
Keywords:
renal cell carcinoma; miR-34a; long-chain non coding RNA
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2022.03.003
Abstract:
Objective:To investigate the mechanism of the interaction between lncRNA-NEAT1 and miR-34a on the growth of RCC.Methods:The expression of miR-34a and NEAT1 in 60 cases of renal carcinoma and adjacent tissues were detected.A498 cells were cultrued stably and trasfect with si-NEAT1.Transient transfection with miR-34a inhibitor was performed by liposome method and the expression of miR-34a or NEAT1 was knocked down to detect the effect on the growth activity of A498 cells.Twenty SD rats were randomly divided into NC group and miR-34a inhibitor group and the differences of various indexes were detected.Results:The expression of lncRNA-NEAT1 was significantly decreased(P＜0.05),and the expression of miR-34a was significantly increased(P＜0.05).After si-NEAT1 transfection,the proliferation,migration and invasion of A498 cells increased significantly,and the apoptosis rate decreased significantly.In si-NEAT1 group,the relative expression of Ki-67 protein and Vimentin protein decreased(P＜0.05),while the expression of Cleaved caspase-3 protein and N-cadherin protein increased(P<0.05).Compared with NC group,the tumor growth of miR-34a inhibitor group was significantly inhibited with a significantly smaller diameter(P＜0.05).HE staining showed that,the number of pulmonary metastasis was less and the diameter was smaller.After go- transfection of miR-34a inhibitor and si-NEAT1,the antitumor effect of si-miR-34a was partially offset,and the cell migration and invasion ability were significantly improved.Compared with the single transfection group,the relative expression of Ki-67 protein，Vimentin protein and N-cadherin protein in go-transfection group was significantly increased(P＜0.05),and the relative expression of Cleaved caspase-3 protein was significantly decreased(P<0.05).Conclusion:lncRNA-NEAT1 can target miR-34a and regulate Vimentin expression,thus inhibiting the growth of renal cell carcinoma,which is expected to become a new therapeutic target.

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Last Update: 2021-12-31