|Table of Contents|

miR-34a enhance the cisplatin sensitivity of nasopharyngeal carcinoma cells by activating AMPK/mTOR pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 03
Page:
402-406
Research Field:
Publishing date:

Info

Title:
miR-34a enhance the cisplatin sensitivity of nasopharyngeal carcinoma cells by activating AMPK/mTOR pathway
Author(s):
LI Xuejuan; MA Fei; MA Qinghua
Department of Otorhinolaryngologic,Zhengzhou Seventh People's Hospital,Henan Zhengzhou 450000,China.
Keywords:
nasopharyngeal carcinoma; microRNA-34a; cisplatin; AMP-activated protein kinase/mammalian target of rapamycin pathway
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2022.03.007
Abstract:
Objective:To investigate the effect of microRNA(miR)-34a on cisplatin sensitivity of nasopharyngeal carcinoma cells and its mechanism.Methods:The expression level of miR-34a in cisplatin resistant HNE1/DDP cells and parent HNE1 cells was detected by real-time fluorescent quantitative PCR. HNE1/DDP cells were divided into non-transfection group(normal culture),miR-NC group(transfection negative control) and miR-34a group(transfection of miR-34a mimic),the expression of miR-34a was detected by real-time fluorescent quantitative PCR,the sensitivity of cells to cisplatin was detected by methyl thiazolyl tetrazolium(MTT) assay,the apoptosis rate was detected by flow cytometry,Western blotting was used to detect the expression levels of apoptosis associated proteins B-lymphoma-2 gene(Bcl-2),Bcl-2-related X protein(Bax),and AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR) pathway proteins AMPK,phosphorylation(p)-AMPK,mTOR and p-mTOR.Results:Compared with that of HNE1 cells,the expression level of miR-34a in HNE1/DDP cells was significantly lower(P<0.05).Compared with that in miR-NC group,miR-34a expression level,apoptosis rate,Bax protein expression level and p-AMPK/AMPK level in miR-34a group were significantly higher,the half inhibitory concentration of cisplatin on cells(IC50),Bcl-2 protein expression level and p-mTOR/mTOR level were significantly lower(P<0.05).Conclusion:Overexpression of miR-34a can enhance the sensitivity of HNE1/DDP cells to cisplatin by inducing apoptosis,which may be related to the activation of AMPK/mTOR pathway.

References:

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Memo

Memo:
河南省医学科技攻关计划联合共建项目(编号：LHGJ20190046)
Last Update: 2021-12-31