|Table of Contents|

Study on the mechanism of miR-4465 suppresses the proliferation，migration and invasion of gastric cancer cells by regulating the expression of EZH2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
22-27
Research Field:
Publishing date:

Info

Title:
Study on the mechanism of miR-4465 suppresses the proliferation，migration and invasion of gastric cancer cells by regulating the expression of EZH2
Author(s):
ZHENG Huashan1; CHEN Chenghui1; CAI Qinping1; LIN Xianke1; LI He2
1.Donghu Surgery,the Second Affiliated Hospital of Hainan Medical College,Hainan Haikou 570100,China;2.General Surgery,the First Affiliated Hospital of Hainan Medical College,Hainan Haikou 570102,China.
Keywords:
miR-4465; EZH2; gastric cancer; proliferation; migration; invasion
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2022.01.005
Abstract:
Objective:To investigate the effect of miR-4465 on the proliferation，migration and invasion of gastric cancer cells by regulating the expression of EZH2.Methods:qRT-PCR was applied to detect the expression level of miR-4465 in gastric cancer tissues and cell lines.Overexpression of miR-4465 or silencing of EZH2 in MKN45 cells,and the proliferation,migration and invasion of MKN45 cells were detected by CCK-8 assay and Transwell assay.Western blotting was used to detect the expression of EZH2,E-cadherin,N-cadherin and Vimentin.The interaction between miR-4465 with EZH2 was verified by dual-luciferase reporter gene.Results:Compared with the adjacent tissues,the expression level of miR-4465 in gastric cancer tissues was decreased,and the expression level in gastric cancer cell lines(MCC-803,SGC7901,MKN45) was significantly lower than that in human immortalized gastric cell RGM-1,and the expression in MKN45 was lower than other cells.Overexpression of miR-4465 significantly inhibits the proliferation,migration,invasion and epithelial-mesenchymal transition of MKN45 cell.Dual-luciferase reporter gene result confirmed that miR-4465 was directly targeted with the 3'-UTR of EZH2.Further experiments showed that simultaneously silencing miR-4465 and EZH2 could restored the inhibitory effect of EZH2 silencing on the proliferation,invasion,migration and EMT of MKN45 cells.Conclusion:miR-4465 is inhibit the biological behavior of gastric cancer cells via targeting EZH2.miR-4465 may be a molecular target for the treatment of gastric cancer.

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Last Update: 2021-12-02