|Table of Contents|

Study on the mechanism of cyanidin-3-O-glucoside enhancing antitumor activiity of human hepatoma HepG2 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
27-32
Research Field:
Publishing date:

Info

Title:
Study on the mechanism of cyanidin-3-O-glucoside enhancing antitumor activiity of human hepatoma HepG2 cells
Author(s):
LI Shuyu1; XUE Hongkun2
1.Department of Rheumatology and Immunology,Shanghai International Medical Center,Shanghai 201318,China;2.Department of Engineering Physics,Tsinghua University,Beijing 100084,China.
Keywords:
cyanidin-3-O-glucoside; HepG2 cell; apoptosis; mechanism
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2022.01.006
Abstract:
Objective:To investigate the mechanism of cyanidin-3-glucoside(C3G) enhance antitumor activity of human hepatoma HepG2 cells.Methods:The MTT method was used to measure the effect of C3G on the inhibition rate of HepG2 cells.The morphology of cells stained with Hoechst 33342 was observed by confocal laser scanning.Meanwhile,the effects of C3G on apoptosis,cell cycle and mitochondrial membrane potential of HepG2 cells were measured by flow cytometry.The effect of C3G on the expression of apoptosis related proteins in HepG2 cells was measured by Western blot.Results:The inhibition rate of HepG2 cells increased significantly with the increase C3G concentration in a concentration dependent manner when the C3G concentration was in the range of 12.5~100.0 μmol/L.At the same time,the IC50 on the inhibition rate of HepG2 cells at 24,48 and 72 h were 97.16，40.35，39.83 μmol/L,respectively.C3G could obviously increase the apoptosis rate and nuclear fluorescence intensity of HepG2 cells,and block the cell cycle in G2/M phase as well as decrease significantly the mitochondrial membrane potential.C3G could up regulate the expression levels of Bax,Cytochrome c,Caspase-9 and Caspase-3 protein and down regulate the expression level of Bcl-2 protein.Conclusion:C3G can achieve antitumor effect by activating mitochondrial mediated apoptosis pathway and cycle arrest.

References:

[1]MA G,CHEN J,WEI T,et al.Inhibiting roles of FOXA2 in liver cancer cell migration and invasion by transcriptionally suppressing microRNA-103a-3p and activating the GREM2/LATS2/YAP axis［J］.Cytotechnology,2021,73(4):523-537.
[2]OKUDA K,UMEMURA A,UMEMURA S,et al.Honokiol prevents non-alcoholic steatohepatitis induced liver cancer via EGFR degradation through the glucocorticoid receptor-mig6 axis［J］.Cancers,2021,13(7):1515.
[3]PORTER A,BARCELON JM,BUDKER RL,et al.Treatment of metastatic placental site trophoblastic tumor with surgery,chemotherapy,immunotherapy and coil embolization of multiple pulmonary arteriovenous fistulate［J］.Gynecologic Oncology Reports,2021,36(6):100782.
[4]TAN C,KONG Y,TONG Y,et al.Anti-apoptotic effects of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on lipopolysaccharide-induced inflammation in Caco-2 cells［J］.Journal of Functional Foods,2021,86(7):104709.
[5]SR A,AJD B,SCD B.In vitro cytoprotective activity of cyanidin 3-glucoside extracts from haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells［J］.Saudi Journal of Biological Sciences,2021,28(9):5338-5348.
[6]张楚阳,张珂嘉,刘琴.矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较［J］.南京工业大学学报(自然科学版),2018,40(3):31-37. ZHANG CY,ZHANG KJ,LIU Q.Comparison of protective effects of cyanidin and cyanidin-3-O-glucoside on oxidative stress induced by H2O2 in HepG-2 cell model［J］.Journal of Nanjing University of Technology(Natural Science Edition),2018,40(3):31-37.
[7]王丽,程鹏,曲晨菲,等.花青素单一成分矢车菊-3-O-葡萄糖苷抑制B16-F10细胞增殖和迁移的机理［J］.生理学报,2019,71(6):855-862. WANG L,CHENG P,QU CF,et al.Mechanism of the anthocyanin single component cyanidin-3-O-glucoside inhibiting proliferation and migration of B16-F10 cells［J］.Journal of Physiology,2019,71(6):855-862.
[8]SUN C,ZHENG Y,CHEN Q,et al.Purification and anti-tumour activity of cyanidin-3-O-glucoside from Chinese bayberry fruit［J］.Food Chemistry,2012,131(4):1287-1294.
[9]CHEN P,CHU SC,CHIOU HL,et al.Cyanidin-3-glucoside and peonidin-3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo［J］.Nutrition and Cancer,2005,53(2):232-243.
[10]HIROTAKA K,HIROSHIGE H,SHINJI O,et al.Cyanidin 3-O-β-D-glucoside isolated from skin of black Glycine max and other anthocyanins isolated from skin of red grape induce apoptosis in human lymphoid leukemia Molt 4B cells［J］.Oncology Reports,2003,10(2):297-300.
[11]SILVEIRA M,PANSA CC,MALASPINA O,et al.The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2:A potential molecular tool for controlling hepatic cellular migration［J］.Molecular Biology Reports,2021,48(4):3463-3474.
[12]LI Y,YAO L,ZHANG L,et al.Enhanced physicochemical stabilities of cyanidin-3-O-glucoside via combination with silk fibroin peptide［J］.Food Chemistry,2021,355:129479.
[13]YIN Z,WU Y,CHEN Y,et al.Analysis of the interaction between cyanidin-3-O-glucoside and casein hydrolysates and its effect on the antioxidant ability of the complexes［J］.Food Chemistry,2020,340:127915.
[14]BLANCH GP,CASTILLO M.Effect of baking temperature on the phenolic content and antioxidant activity of black corn(Zea mays L.) bread［J］.Foods,2021,10(6):1202.
[15]WEI T,JI X,XUE J,et al.Cyanidin-3-O-glucoside represses tumor growth and invasion in vivo by suppressing autophagy via inhibition of the JNK signaling pathways［J］.Food & Function,2021,12(1):387-396.
[16]SUN CD,ZHENG YX,CHEN QJ,et al.Purification and anti-tumour activity of cyanidin-3-O-glucoside from Chinese bayberry fruit［J］.Food Chemistry,2012,131(4):1287-1294.
[17]LIANG T,GUAN R,WANG Z,et al.Comparison of anticancer activity and antioxidant activity between cyanidin-3-O-glucoside liposomes and cyanidin-3-O-glucoside in Caco-2 cells in vitro［J］.RSC advances,2017,7(59):37359-37368.
[18]李新.黑豆皮花青素性质及抑制肿瘤细胞增殖的研究［D］.太原：山西大学,2015. LI X.Study on the properties of anthocyanins from black soybean coats and inhibition of the proliferation of tumor cells［D］.Taiyuan:Shanxi University,2015.
[19]YU TJ,CHENG YB,LIN LC,et al.Physalis peruviana-derived physapruin a(PHA) inhibits breast cancer cell proliferation and induces oxidative-stress-mediated apoptosis and DNA damage［J］.Antioxidants,2021,10(3):393.
[20]FU Y,RICCIARDIELLO F,YANG G,et al.The role of mitochondria in the chemoresistance of pancreatic cancer cells［J］.Cells,2021,10(3):497.
[21]WANG X,GONG Q,SONG C,et al.Berberine-photodynamic therapy sensitizes melanoma cells to cisplatin-induced apoptosis through ROS-mediated p38 MAPK pathways［J］.Toxicology and Applied Pharmacology,2021,418:115484.
[22]马汉宁,姬艳燕,陈伟,等.甘草次酸通过抑制Caspase 3/Bax/Bcl-2凋亡信号通路保护心脏骤停心肺复苏大鼠心脏功能［J］.中药药理与临床,2019,35(4):28-33. MA HN,JI YY,CHEN W,et al.Effects of astragaloside on calcium-phosphorus metabolism in rats with uremic peritoneal dialysis by regulating Caspase 3/Bax/Bcl-2 pathway［J］.Pharmacology and Clinics of Chinese Materia Medica,2019,35(4):28-33.
[23]ZHAN R,GUO W,GAO X,et al.Real-time in situ monitoring of Lon and Caspase-3 for assessing the state of cardiomyocytes under hypoxic conditions via a novel Au-Se fluorescent nanoprobe［J］.Biosensors & Bioelectronics,2021,176(3):112965.

Memo

Memo:
中国博士后基金面上资助项目（编号：2020M670357）
Last Update: 2021-12-02