|Table of Contents|

Study on the mechanism of puerarin in inhibiting the proliferation,invasion and migration of non-small cell lung cancer A549 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
16-21
Research Field:
Publishing date:

Info

Title:
Study on the mechanism of puerarin in inhibiting the proliferation,invasion and migration of non-small cell lung cancer A549 cells
Author(s):
LI Tao1; 2; 3; GONG Changzhi3; ZHU Jiabin2; 4; CHEN Huan2; SHANG Fajun4
Keywords:
non-small cell lung cancer; puerarin; proliferation; invasion; migration; NF-κB
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2022.01.004
Abstract:
Objective:To investigate the effect of puerarin on the proliferation,invasion and migration of non-small cell lung cancer A549 cells and its mechanism.Methods:After treating non-small cell lung cancer A549 cells with different concentrations of puerarin,CCK-8 was used to detect the inhibitory effect of puerarin on the proliferation of non-small cell lung cancer A549 cells and the IC50.Microscope was used to observe the effect of puerarin on the cell morphology of non-small cell lung cancer A549 cells.Plate cloning experiment was used to detect the effect of puerarin on the growth of non-small cell lung cancer A549 cells in vitro.Annexin V/PI double staining flow cytometry was used to detect lung cancer cell apoptosis rate.Transwell test and scratch test were used to detect the effect of puerarin on the invasion and migration ability of non-small cell lung cancer A549 cells.Western blot test was used to detect the effect of puerarin on MMP-2,MMP-9,p-p65,p65 protein expression of non-small cell lung cancer A549 cells.Results:Compared with the control group,puerarin inhibited the cell viability of A549 cells in a dose-dependent manner(0.8 mmol/L,1.6 mmol/L,3.2 mmol/L)(P＜0.05),and the IC50 of puerarin after acting on A549 cells for 48 hours was (1.917±0.119) mmol/L.Puerarin can significantly change the morphology of A549 cells,which is manifested as a decrease in cell number,shrinkage and rounding of cells,weakened cell attachment ability,increased cell spacing,and decreased filopodia.Compared with the control group,both 2 mmol/L and 3 mmol/L puerarin can significantly inhibit the colony forming ability of A549 cells(P＜0.05).Compared with the control group,puerarin induced A549 cell apoptosis in a dose-dependent manner(1 mmol/L,2 mmol/L,3 mmol/L)(P＜0.05).Compared with the control group,both 2 mmol/L and 3 mmol/L puerarin can significantly inhibit the invasion ability of A549 cells(P＜0.05).Compared with the control group,puerarin inhibited the migration ability of A549 cells in a dose-dependent manner(1 mmol/L,2 mmol/L,3 mmol/L)(P＜0.05).Compared with the control group,puerarin inhibited the expression of MMP-2 protein,MMP-9 protein and p-p65/p65 in A549 cells in a dose-dependent manner(1 mmol/L,2 mmol/L,3 mmol/L)(P＜0.05).Conclusion:Puerarin may inhibit the proliferation,invasion and migration of lung cancer A549 cells through the NF-κB signaling pathway.

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Memo

Memo:
湖北省卫生健康科研基金（编号：WJ2019H544）；湖北省宜昌市医疗卫生研究项目(编号：A20-2-046);肿瘤微环境与免疫治疗湖北省重点实验室（三峡大学）开放基金（编号：2019KZL07）
Last Update: 2021-12-02