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The expression and significance of ACY-1 in the tissues and cells of children with neuroblastoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
11-15
Research Field:
Publishing date:

Info

Title:
The expression and significance of ACY-1 in the tissues and cells of children with neuroblastoma
Author(s):
CHEN Zimin; SUN Jun; GAO Jiahui; WU Zhouguang; ZHANG Xiaowen; WANG Zihan
Department of Pediatric General Surgery Ⅱ，Shenzhen Children's Hospital，Guangdong Shenzhen 518038，China.
Keywords:
ACY-1; children; neuroblastoma
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2022.01.003
Abstract:
Objective:To investigate the expression and significance of aminoacylase-1(ACY-1) in the tissues and cells of children with neuroblastoma.Methods:qRT-PCR and Western blot were used to detect the expression of ACY-1 in tumor tissues and adjacent tissues of children with neuroblastoma.Then,in vitro experiments were conducted on human neuroblastoma cells(SH-SY5Y cell line) to overexpress or inhibit the expression of ACY-1 by using recombinant adenovirus vector and transfection technology.The effects of ACY-1 on the proliferation,apoptosis,migration and invasion of the tumor cells were detected by CCK-8 method,flow cytometry and Transwell method.Results:The results of qRT-PCR and Western blot showed that the mRNA and protein expression levels of ACY-1 in tumor tissues were lower than those in the adjacent tissues.ACY-1 overexpression not only inhibited the proliferation of SH-SY5Y cell line and induced its apoptosis,but also inhibited its migration and invasion.Inhibition of the expression of ACY-1 showed the opposite result.Conclusion:ACY-1 may be closely related to the occurrence and development of neuroblastoma in children,and it can regulate the proliferation,apoptosis,migration and invasion of neuroblastoma cells.

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Memo

Memo:
广东省医学科学技术研究基金项目（编号：A2020562）；广东省深圳市医疗卫生三名工程（编号：SZSM201812055）
Last Update: 2021-12-02