|Table of Contents|

Long noncoding RNA LINC-PINT regulates laryngeal carcinoma cell stemness through miR-425-5p/PTCH1 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
6-10
Research Field:
Publishing date:

Info

Title:
Long noncoding RNA LINC-PINT regulates laryngeal carcinoma cell stemness through miR-425-5p/PTCH1 axis
Author(s):
YU Pingyang; YUAN Zhennan; SUN Ji
Department of Otorhinolaryngology Head and Neck Surgery,Harbin Medical University Cancer Hospital,Heilongjiang Harbin 150081,China.
Keywords:
laryngeal tumors; LINC-PINT; miR-425-5p; PTCH1; cancer cell stemness
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2022.01.002
Abstract:
Objective:To investigate the effect of long noncoding RNA(lncRNA) LINC-PINT/miR-425-5p/PTCH1 axis on laryngeal cancer stemness.Methods:The full-length sequences of LINC-PINT were amplified and transfected into HEp-2 cells using polymerase chain reaction(qRT-PCR) methods.Predict and verify the molecular targets of LINC-PINT and miR-425-5p using Diana Tools and dual luciferase report assay system respectively.qRT-PCR and Western blot were used to detect the expression of LINC-PINT and cancer stemness-associated genes(Sox-2,CD44,CD133 and Nanog) and their proteins.Results:After transfection of LINC-PINT,it was confirmed that the overexpression of LINC-PINT would cause the down-regulation of the expression of cancer stem-associated genes.Inhibition of miR-425-5p can down-regulate the expression of cancer stemness-associated genes.LINC-PINT regulated miR-425-5p and miR-425-5p targeted PTCH1.Conclusion:LINC-PINT may contribute to laryngeal carcinoma through inhibiting cancer cell stemness by miR-425-5p/PTCH1 axis.

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Memo

Memo:
黑龙江省卫生健康委科研课题（编号：2019-054）;哈尔滨医科大学附属肿瘤医院海燕科研基金（编号：JJQN2019-05）
Last Update: 2021-12-02