|Table of Contents|

Detection of extramedullary (except CSF) involvement of acute leukaemia using flow cytometry

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 06
Page:
1024-1028
Research Field:
Publishing date:

Info

Title:
Detection of extramedullary (except CSF) involvement of acute leukaemia using flow cytometry
Author(s):
WANG Hui1; WANG Aixian1; ZHEN Junyi1; WU Xueying1; CHEN Man1; GONG Meiwei1; WU Ping1; CHANG Yingjun2
1.Department of Clinical Laboratory,Hebei Yanda Ludaopei Hospital,Hebei Langfang 065201,China;2.Hematological Institute,People's Hospital of Peking University,Beijing 100044,China.
Keywords:
flow cytometry; extramedullary; acute leukaemia
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2021.06.026
Abstract:
Objective:To study the value of flow cytometry in identifying extramedullary （except cerebro-spinal fluid） involvement of acute leukaemia（AL）.Methods：In the patients who were diagnosed and treated in Hebei Yanda Ludaopei Hospital from Oct.2008 to Dec.2014,extramedullary samples from acute leukemia patients were detected by flow cytometry.Results：Malignant cells were found in 59 samples.The median percentage of tumor cells was 41.93%（0.38%～97.87%）.The immunophenotyping of bone marrows(BM) from 51 patients were detected at the same time,of which 29(57%) had concurrent BM diseases.The median percentage of tumor cells was 10.91%（0.02%～85.19%）in BM.Patients after allogeneic hematopoietic stem cell transplantation(allo-SCT) had lower frequency of concurrent BM diseases(P＜0.001).Comparing to acute myeloid leukaemia(AML),acute lymphoblastic leukaemia（ALL）had higher frequency of solid extramedullary involvement（P＜0.05）.In AML,French-American-British classification M4/M5 leukemia and CD56 positive AML were proned to having extramedullary involvement（P＜0.01,P＜0.001,respectively）.Conclusion：Extramedullary (except cerebro-spinal fluid) involvement of AL could be effectively detected by flow cytometry.

References:

［1］SHEM-TOV N,SARACENI F,DANYLESKO I,et al.Isolated extramedullary relapse of acute leukemia after allogeneic stem cell transplantation:different kinetics and better prognosis than systemic relapse［J］.Biol Blood Marrow Transplant,2017,23(7):1087-1094.
[2]MO XD,KONG J,ZHAO T,et al.Extramedullary relapse of acute leukemia after haploidentical hematopoietic stem cell transplantation:incidence,risk factors,treatment,and clinical outcomes［J］.Biol Blood Marrow Transplant,2014,20(12):2023-2028.
[3]GE L,YE F,MAO X,et al.Extramedullary relapse of acute leukemia after allogeneic hematopoietic stem cell transplantation:different characteristics between acute myelogenous leukemia and acute lymphoblastic leukemia［J］.Biol Blood Marrow Transplant,2014,20(7):1040-1047.
[4]STOVE HK,SANDAHL JD,ABRAHAMSSON J,et al.Extramedullary leukemia in children with acute myeloid leukemia:A population-based cohort study from the Nordic Society of Pediatric Hematology and Oncology (NOPHO)［J］.Pediatr Blood Cancer,2017,64(12):26520.
[5]YUDA S,FUJI S,ONISHI A,et al.Extramedullary relapse of acute myelogenous leukemia after allogeneic hematopoietic stem cell transplantation［J］.Biol Blood Marrow Transplant,2019,25(6):1152-1157.
[6]ULLMAN DI,DORN D,JONES JA,et al.Clinicopathological and molecular characteristics of extramedullary acute myeloid leukaemia［J］.Histopathology,2019,75(2):185-192.
[7]SOLH M,SOLOMON S,MORRIS L,et al.Extramedullary acute myelogenous leukemia［J］.Blood Rev,2016,30(5):333-339.
[8]LAZZAROTTO D,CANDONI A,FIL C,et al.Clinical outcome of myeloid sarcoma in adult patients and effect of allogeneic stem cell transplantation.Results from a multicenter survey［J］.Leuk Res,2017,53:74-81.
[9]PARK SS,YOON JH,KIM HJ,et al.Characteristics and survival outcome analysis of extramedullary involvement in adult patients with t(8;21) acute myeloid leukemia［J］.Clin Lymphoma Myeloma Leuk,2017,17(1):38-45.
[10]ALAKEL N,STLZEL F,MOHR B,et al.Symptomatic central nervous system involvement in adult patients with acute myeloid leukemia［J］.Cancer Manag Res,2017,9:97-102.
[11]BENTO LC,CORREIA RP,ALEXANDRE AM,et al.Detection of central nervous system infiltration by myeloid and lymphoid hematologic neoplasms using flow cytometry analysis:diagnostic accuracy study［J］.Front Med (Lausanne),2018,5:70.
[12]THASTRUP M,MARQUART HV,LEVINSEN M,et al.Flow cytometric detection of leukemic blasts in cerebrospinal fluid predicts risk of relapse in childhood acute lymphoblastic leukemia:a Nordic Society of Pediatric Hematology and Oncology study［J］.Leukemia,2020,34(2):336-346.
[13]TAWFIK B,BROWN L,FUDA F,et al.Utility and proposed algorithm of CSF flow cytometry in hematologic malignancies［J］.Ann Hematol,2018,97(9):1707-1716.
[14]KOVACH AE,DELELYS ME,KELLIHER AS,et al.Diagnostic utility of cerebrospinal fluid flow cytometry in patients with and without prior hematologic malignancy［J］.Am J Hematol,2014,89(10):978-984.
[15]ARBER DA,ORAZI A,HASSERJIAN R,et al.The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia［J］.Blood,2016,127(20):2391-2405.
[16]卢岳，吴彤，王卉,等.预处理前多参数流式细胞术监测的微小残留病对急性髓系白血病异基因造血干细胞移植预后的影响［J］.中华血液学杂志，2017,38(2)：118-123. LU Y,WU T,WANG H,et al.Effect of minimal residual disease monitoring by multiparameter flow cytometry pre-conditioning on prognosis of acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation［J］.Chinese Journal of Hematology，2017,38(2)：118-123.
[17]GABELLI M,ZECCA M,MESSINA C,et al.Hematopoietic stem cell transplantation for isolated extramedullary relapse of acute lymphoblastic leukemia in children［J］.Bone Marrow Transplant,2019,54(2):275-283.
[18]HAGEDORN N,ACQUAVIVA C,FRONKOVA E,et al.Submicroscopic bone marrow involvement in isolated extramedullary relapses in childhood acute lymphoblastic leukemia:a more precise definition of "isolated" and its possible clinical implications,a collaborative study of the Resistant Disease Committee of the International BFM study group［J］.Blood,2007,110:4022-4029.
[19]KIYOHARA T,MAKIMURA K,NAKAMARU S,et al.B-cell lymphoblastic leukemia/lymphoma in infants:Report of two cases on the face［J］.J Dermatol,2019,46(7):e255-e256.
[20]SILVA WFD,MARQUEZ GL,SALIM RC,et al.Primary refractory B-cell lymphoblastic leukemia with extramedullary disease - a distinctive response to blinatumomab and inotuzumab ozogamicin［J］.Hematol Transfus Cell Ther,2019,41(4):356-359.
[21]ROBILLARD DT,KUTNY MA,CHEWNING JH,et al.Extramedullary relapse of acute lymphoblastic leukemia presenting as abnormal uterine bleeding:a case report［J］.J Pediatr Adolesc Gynecol,2017,30(3):431-434.
[22]LIU B,LIU B,WANG X,et al.Complete response of extramedullary relapse in breast of acute T lymphoblastic leukemia after bone marrow transplantation to chemoradiotherapy:a case report and literature review［J］.BMC Cancer,2016,16(1):875.
[23]DE HAAS V,ISMAILA N,ADVANI A,et al.Initial diagnostic work-up of acute leukemia:ASCO clinical practice guideline endorsement of the College of American Pathologists and American Society of Hematology guideline［J］.J Clin Oncol,2019,37(3):239-253.
[24]MARTINEZ A,AYMERICH M,CASTILLO M,et al.Routine use of immunophenotype by flow cytometry in tissues with suspected hematological malignancies［J］.Cytometry Part B (Clinical Cytometry),2003,56B:8-15.
[25]DEMURTAS A,STACCHINI A,ALIBERTI S,et al.Tissue flow cytometry immunophenotyping in the diagnosis and classification of non-Hodgkin's lymphomas:a retrospective evaluation of 1,792 cases［J］.Cytometry B Clin Cytom,2013,84(2):82-95.
[26]WAKE LM,VANDEN BUSSCHE CJ,ALI SZ,et al.Flow cytometric analysis of fine needle aspirates is affected by tumor subtype,but not by anatomic location nor technique［J］.Diagn Cytopathol,2020,48(6):538-546.
[27]PRADIER A,TABONE-EGLINGER S,HUBER V,et al.Peripheral blood CD56(bright) NK cells respond to stem cell factor and adhere to its membrane-bound form after upregulation of c-kit［J］.Eur J Immunol,2014,44(2):511-520.
[28]TROPEA MM,HARPER BJ,GRANINGER GM,et al.Isolation of a circulating CD45-,CD34dim cell population and validation of their endothelial phenotype［J］.Thromb Haemost,2014,112(4):770-780.

Memo

Memo:
京津冀基础研究合作专项（编号：H2018206548）
Last Update: 1900-01-01