|Table of Contents|

Influence of B7-H3 silencing on epithelial-mesenchymal transition and biological function of breast cancer MDA-MB-231 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 06
Page:
919-924
Research Field:
Publishing date:

Info

Title:
Influence of B7-H3 silencing on epithelial-mesenchymal transition and biological function of breast cancer MDA-MB-231 cells
Author(s):
CHEN Xi; WANG Huiping; ZHANG Zhe; ZHANG Huizhong; DONG Ke
Department of Clinical Diagnosis,Tangdu Hospital,Air Force Medical University,Shaanxi Xi'an 710038,China.
Keywords:
B7-H3; breast cancer; epithelial to mesenchymal transition; cell metastasis; cell proliferation
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2021.06.003
Abstract:
Objective:To investigate the effect of B7-H3 silencing on epithelial-mesenchymal transition (EMT) and biological behaviors of human breast cancer MDA-MB-231 cells.Methods:LipofectamineTM2000 were used to transfect B7-H3 siRNA vector pSilencer4.1-CMV neo/B7-H3 into breast cancer MDA-MB-231 cells，which was subsequently screened by G418 in order to acquire positive cell model whose B7-H3 level was decreased.qRT-PCR and Western blot were used to detect the mRNA and protein levels of B7-H3 and EMT marker molecules in control groups (WT),non-specific interference groups (ShNC) and B7-H3 interference groups (ShB7-H3).Transwell experiments were also used to detect cell migration and invasion.MTT assay was applied to evaluate cell proliferation.Results:The results of RT-PCR and Western blot demonstrated that the expression of B7-H3 was largely knocked down in MDA-MB-231 cells.Compared with the control groups,the EMT process of model cells with B7-H3 silencing were suppressed,accompanied by E-cadherin and ZO-1 were significantly up-regulated while vimentin and αSMA were down-regulated.Transwell experiments and MTT assays showed that the silent expression of B7-H3 inhibited cell migration,invasion and proliferation.Conclusion:Our present results emphasize the important role of B7-H3 in human breast cancer MDA-MB-231 cells,and suggest that B7-H3 is closely related with breast cancer EMT and metastasis,which lay good foundation concerning experiment and theory for exploring breast cancer metastasis therapy aiming at B7-H3.

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Memo

Memo:
National Natural Science Foundation of China(No.81772485）；国家自然科学基金资助项目（编号：81772485）
Last Update: 1900-01-01