|Table of Contents|

miR-409-3p inhibits proliferation,migration and invasion of nasopharyngeal carcinoma cells by targeted regulation of RRM2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 06
Page:
912-918
Research Field:
Publishing date:

Info

Title:
miR-409-3p inhibits proliferation,migration and invasion of nasopharyngeal carcinoma cells by targeted regulation of RRM2
Author(s):
XIE Chen; YAO Mengxian; CAO Zhongsheng; TIAN Ye
The Second Affiliated Hospital of Soochow University,Jiangsu Suzhou 215000,China.
Keywords:
miR-409-3p; RRM2; nasopharyngeal carcinoma; proliferation; migration; invasion
PACS:
R739.63
DOI:
10.3969/j.issn.1672-4992.2021.06.002
Abstract:
Objective:To investigate the effect of microRNA-409-3p (miR-409-3p) on proliferation,migration and invasion of nasopharyngeal carcinoma cells and its mechanism.Methods:The expressions of miR-409-3p and RRM2 in NP69,5-8F and 6-10B cells were detected by qRT-PCR and Western blot,respectively.The cell counting kit (CCK-8) detects the effect of miR-409-3p overexpression or RRM2 overexpression on 6-10B cell proliferation.The Transwell chamber assay detects the effects of miR-409-3p overexpression or RRM2 overexpression on 6-10B cell migration and invasion.Dual luciferase reporter assay and Western blot assay for the regulation of miR-409-3p and RRM2 in 6-10B cells.Western blot was used to detect the expression of c-Myc,CyclinD1,MMP-2 and MMP-9 proteins.Results:Compared with NP69 cells and 5-8F cells,the expression level of miR-409-3p was significantly decreased in 6-10B cells (P＜0.05),and the expression level of RRM2 was significantly increased (P＜0.05).The cell survival rate of miR-409-3p was significantly decreased (P＜0.05),the number of migrated cells and the number of invasive cells were significantly decreased (P＜0.05),and the expression levels of c-Myc,CyclinD1,MMP-2 and MMP-9 were significantly decreased (P＜0.05).The dual luciferase reporter assay confirmed that miR-409-3p can bind to RRM2,and the expression of RRM2 in 6-10B cells was down-regulated after enhancing the expression of miR-409-3p (P＜0.05).Overexpression of RRM2 significantly attenuated the inhibitory effect of miR-409-3p overexpression on the proliferation,migration and invasion of 6-10B cells (P＜0.05).Conclusion:miR-409-3p may inhibit the proliferation,migration and invasion of nasopharyngeal carcinoma cells by negatively regulating the expression of RRM2.

References:

［1］张欣,郑美娟,徐元宏.miRNA-143对人鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响［J］.安徽医科大学学报,2017,52(4):480-484. ZHANG X,ZHENG MJ,XU YH.Effect of miRNA-143 on proliferation,migration and invasion of CNE-2Z cells of human nasopharyngeal cancer［J］.Acta Universitatis Medicinalis Anhui,2017,52(4):480-484.
［2］ZHANG W,CHEN Y,CHEN L,et al.The clinical utility of plasma Epstein-Barr virus DNA assays in nasopharyngeal carcinoma:the dawn of a new era?:a systematic review and meta-analysis of 7836 cases［J］.Medicine (Baltimore),2015,94(20):e845-e855.
［3］SONG YL,PAN MZ,WANG AL.MicroRNA-646 inhibits proliferation and cell cycle progression of nasopharyngeal carcinoma cells by targeting mTOR［J］.Eur Rev Med Pharmacol Sci,2019,23(20):8905-8912.
［4］MAI S,XIAO R,SHI L,et al.MicroRNA-18a promotes cancer progression through SMG1 suppression and mTOR pathway activation in nasopharyngeal carcinoma［J］.Cell Death Dis,2019,10(11):819-830.
［5］ZHANG G,LIU Z,XU H,et al.miR-409-3p suppresses breast cancer cell growth and invasion by targeting Akt1［J］.Biochem Biophys Res Commun,2016,469(2):189-195.
［6］WU L,ZHANG Y,HUANG Z,et al.MiR-409-3p inhibits cell proliferation and invasion of osteosarcoma by targeting zinc-finger E-Box-binding homeobox-1［J］.Front Pharmacol,2019,10(1):137-148.
［7］HAN P,LIN ZR,XU LH,et al.Ribonucleotide reductase M2 subunit expression and prognostic value in nasopharyngeal carcinoma［J］.Mol Med Rep,2015,12(1):401-409.
［8］张磊,徐巍.鼻咽癌的治疗进展［J］.国际肿瘤学杂志,2017,44(9):693-695. ZHANG L,XU W.Progress in the treatment of nasopharyngeal carcinoma［J］.Journal of International Oncology,2017,44(9):693-695.
［9］闫勇,吴曙辉,朱静静,等.miR-133b靶向下调EGFR对鼻咽癌SUNE-1细胞增殖、迁移及侵袭的影响［J］.贵州医科大学学报,2018,43(12):1420-1435. YAN Y,WU SH,ZHU JJ,et al.Inhibitory effect of miR-133b on down-regulation of EGFR expression in nasopharyngeal carcinoma SUNE-1 cells［J］.Journal of Guizhou Medical University,2018,43(12):1420-1435.
［10］佘笠,谭浩蕾,朱刚才,等.MiR-98靶向MTDH调控鼻咽癌恶性进展的实验研究［J］.中国耳鼻咽喉颅底外科杂志,2016,22(6):455-461. SHE L,TAN HL,ZHU GC,et al.MiR-98 regulates the malignant progression of nasopharyngeal carcinoma via targeting MTDH［J］.Chinese Journal of Otorhinolaryngology-skull Base Surgery,2016,22(6):455-461.
［11］张国强,刘增艳,王晓红,等.血清微小RNA-409-3p表达在乳腺癌早期诊断中的价值［J］.江苏医药,2018,44(4):381-384. ZHANG GQ,LIU ZY,WANG XH,et al.Value of serum microRNA-409-3p expression in early diagnosis of breast cancer［J］.Jiangsu Medical Journal,2018,44(4):381-384.
［12］邓应君,张相良.MicroRNA-409-3p在恶性肿瘤中表达和调控的研究进展［J］.中华生物医学工程杂志,2017,23(5):430-433. DENG YJ,ZHANG XL.Research progress of microRNA-409-3p expression and regulation in malignant tumors［J］.Chinese Journal of Biomedical Engineering,2017,23(5):430-433.
［13］MA Z,LI Y,XU J,et al.MicroRNA-409-3p regulates cell invasion and metastasis by targeting ZEB1 in breast cancer［J］.IUBMB Life,2016,68(5):394-402.
［14］任宗娜.沉默Notch4基因对乳腺癌细胞系MDA-MB-231增殖和迁移侵袭能力的影响［J］.天津医科大学学报,2015,21(6):469-473. REN ZN.Inhibition effect of silencing Notch4 gene on the proliferation and migration and invasion activity of breast cancer cell line MDA-MB-231［J］.Journal of Tianjin Medical University,2015,21(6):469-473.
［15］张萌,张向东,夏永欣,等.钙周期素结合蛋白促进胃癌细胞迁移侵袭和MMP-2、MMP-9、p-ERK1/2、p-AKT水平上调［J］.中国组织化学与细胞化学杂志,2019,28(2):134-141. ZHANG M,ZHANG XD,XIA YX,et al.Calcyclin binding protein promotes the migration and invasion of gastric cancer cells and up-regulates levels of MMP-2,MMP-9,p-ERK1/2 and p-AKT［J］.Chinese Journal of Histochemistry and Cytochemistry,2019,28(2):134-141.
［16］王琪,冯辉,王海冀,等.利用公共数据集分析RRM2在肺癌中的表达及临床意义［J］.肿瘤学杂志,2018,24(7):666-671. WANG Q,FENG H,WANG HJ,et al.Analysis of clinical significance of expression level of ribonucleotide reductase regulatory subunit M2 among lung cancer patients based on GEO datasets［J］.Journal of Chinese Oncology,2018,24(7):666-671.
［17］陈辉,瞿丽,黄永刚,等.小干扰RNA特异靶向沉默RRM2基因对人肝癌细胞HepG2的生物学影响［J］.中华实验外科杂志,2012,29(10):1917-1920. CHEN H,ZHAI L,HUANG YG,et al.Effect of silencing RRM2 by specific small interference RNA on biological behaviors of human hepatoma cancer cell line HepG2［J］.Chinese Journal of Experimental Surgery,2012,29(10):1917-1920.
［18］MAZZU YZ,ARMENIA J,CHAKRABORTY G,et al.A novel mechanism driving poor-prognosis prostate cancer:Overexpression of the DNA repair gene,ribonucleotide reductase small subunit M2 (RRM2)［J］.Clin Cancer Res,2019,25(14):4480-4492.

Memo

Memo:
江苏省卫生计生委课题基金（编号：CXTDA2017037）
Last Update: 1900-01-01