|Table of Contents|

Expressions and significances of miR-145 and KLF5 in bone marrow fluid of multiple myeloma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
467-471
Research Field:
Publishing date:

Info

Title:
Expressions and significances of miR-145 and KLF5 in bone marrow fluid of multiple myeloma
Author(s):
LIU Chuancai; CHEN Wenxi; FU Xianjie
Department of Digestive Hematology，Ezhou Central Hospital，Hubei Ezhou 436000，China.
Keywords:
microRNA-145; Krüepple-like factor 5; multiple myeloma; expression
PACS:
R733.3
DOI:
10.3969/j.issn.1672-4992.2021.03.023
Abstract:
Objective：To study the expressions of microRNA-145(miR-145) and KLF5 in bone marrow fluid of multiple myeloma and their clinical significances.Methods：From January 2015 to March 2016，65 patients with multiple myeloma(MM) and 36 healthy subjects(control group) were selected as subjects.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) was used to detect the expression levels of miR-145 and KLF5 mRNA.Western Blot was used to detect the expression of KLF5 protein.The correlation between the expressions of miR-145 and KLF5 in multiple myeloma bone marrow fluid was analysed.Results：The expression of miR-145 in MM bone marrow fluid was significantly lower than that in normal control group(P<0.05).The expressions of KLF5 mRNA and protein were significantly higher than those in the control group(P<0.05).Compared with phase I and phase Ⅱ，the expression of miR-145 in bone marrow fluid of phase Ⅲ was significantly decreased(P<0.05)，and the expression of KLF5 mRNA was significantly increased(P<0.05).Compared with IgG and IgA，the relative expression level of miR-145 in bone marrow fluid of light chain and non-secretory patients was significantly lower(P<0.05).There was no significant difference in the relative expression level of KLF5 in different immunophenotypes(P>0.05).The expression of miR-145 was negatively correlated with that of KLF5 mRNA(r=-0.452，P<0.05).The expression of miR-145 in bone marrow fluid of dead MM patients was significantly lower than that of survivors(P<0.05),and the expression of KLF5 was significantly higher than that of survival patients(P<0.05).Conclusion：The expression of miR-145 in bone marrow fluid of patients with MM is low，KLF5 is highly expressed,which may be closely related to the occurrence and development of multiple myeloma.

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Last Update: 2020-12-31