|Table of Contents|

Differential expression profile and bioinformatic analysis of urine exosomal miRNA in bladder cancer patients

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
446-450
Research Field:
Publishing date:

Info

Title:
Differential expression profile and bioinformatic analysis of urine exosomal miRNA in bladder cancer patients
Author(s):
DENG Yisen1; 2; HE Yuhui1; 2; YING Wenwei1; 2; LIU Hailong1; 2; LI Peizhe1; 2; ZHOU Xiaofeng1; 2
1.Department of Urology，China-Japan Friendship Hospital，Beijing 100029，China；2.Peking University China-Japan Friendship School of Clinical Medicine，Beijing 100029，China.
Keywords:
bladder cancer; urine; exosome; miRNA; bioinformatic analysis
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2021.03.018
Abstract:
Objective：To detect and screen differentially expressed miRNAs in urine exosomes of bladder cancer patients and normal humans，and to investigate the role of miRNAs in the pathogenesis of bladder cancer.Methods：Ultracentrifugation and Illumina high-throughput sequencing were used to separate and detect the expression of exosomal miRNA in urine samples from 5 bladder cancer patients and 5 matched individuals，and to construct differential expression profiles.Bioinformatic analysis was done to find the major biological functions of differentially expressed microRNAs and their possible signaling pathways.Results：Compared with normal subjects，there were 38 significantly differentially expressed miRNAs in urine exosomes of patients with bladder cancer，of which 34 were up-regulated and 4 were down-regulated.Through GO(Gene Ontology) enrichment and KEGG(Kyoto Encyclopedia of Genes and Genomes) analysis，the miRNAs may participate in the progression of bladder cancer through chemokine signaling pathway，PI3K-Akt signaling pathway，MAPK signaling pathway and RAS signaling pathway.Besides，they involved in lipid metabolism，polysaccharides degradation，RNA degradation and base excision repair and other biochemical metabolic pathways，and participated in a variety of biological processes such as cell adhesion，endocytosis and synaptic vesicle circulation.Conclusion：The expression profile of miRNA in urinary exosomes of bladder cancer patients has changed ramarkably.miRNAs such as has-miR-486-5p，hsa-miR-199b-5p，hsa-miR-7704 and has-miR-184 may play an important role in the development and progression of bladder cancer through chemokine signaling pathway，PI3K-Akt signaling pathway，MAPK signaling pathway and RAS signaling pathway.

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Memo

Memo:
National Natural Science Foundation of China(No.81972403）；国家自然科学基金（编号：81972403）；国家国际科技合作专项（编号：2014DFE30010）
Last Update: 2020-12-31