|Table of Contents|

Comparison of analgesic effect of zoledronic acid in different administration methods on bone cancer pain rats

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
375-380
Research Field:
Publishing date:

Info

Title:
Comparison of analgesic effect of zoledronic acid in different administration methods on bone cancer pain rats
Author(s):
GUO Chihua; ZHAO Yan; LIU Huan; GUO Yufang; WANG Xiang; WANG Shuang; XU Hao
School of Basic Medical Sciences，Xi'an Medical University，Shaanxi Xi'an 710021，China.
Keywords:
bone cancer pain; zoledronic acid; analgesia; microglia activation
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2021.03.003
Abstract:
Objective：To explore the analgesic effect and mechanism of zoledronic acid (ZOL) on bone cancer pain rats by intrathecal injection (i.t.) and subcutaneous injection (s.c.).Methods：Bone cancer pain (BCP) was induced by injection of Walker 256 mammary gland carcinoma cells into the tibia of female SD rats.The Hargreaves test was applied to compare two administrations of ZOL on bone cancer pain behavior of rats after single or continuous administration.RT-PCR，immunofluorescence staining and Western blot analysis were used to observe the changes of IBA-1 in the spinal dorsal horn.Rotarod experiment and open field test were used to detect the effects of ZOL in the motor function and locomotor activity of rats.Results：Single or continuous i.t.or s.c.injection of ZOL could significantly improve the thermal hyperalgesia of rats (P<0.05)，and the analgesic effect of i.t.injection was better than that of s.c.injection (P<0.05).RT-PCR，immunofluorescence staining and Western blot analysis showed that the overexpression of IBA-1 was significantly inhibited after i.t.continuous injection of ZOL for 7 days in bone cancer pain rats (P<0.05)，but continuous s.c.injection of ZOL had no such inhibitory effect (P>0.05).i.t.or s.c.injection of ZOL did not affect the motor function of rats (P>0.05).Conclusion：Compared with s.c.injection of ZOL，i.t.injection of ZOL has stronger analgesic effect in bone cancer pain rats，and inhibition of activation of spinal microglia may be one of its mechanisms，in addition，zoledronic acid exerts analgesic effect without any obvious measurable neurotoxic effects.

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Memo

Memo:
National Natural Science Foundation of China（No.81803099）;国家自然科学基金项目（编号：81803099）
Last Update: 2020-12-31