|Table of Contents|

lncRNA LUADT1 targets miR-138-5p to regulate proliferation，migration and invasion of glioma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
370-375
Research Field:
Publishing date:

Info

Title:
lncRNA LUADT1 targets miR-138-5p to regulate proliferation，migration and invasion of glioma cells
Author(s):
WU Ke1; ZHANG Junping2; WANG Daiwen3; XUE Fengjun2; TANG Shujun1; SONG Chen1; WEI Jianbo1
1.Department of Neurosurgery，Panzhihua Central Hospital，Sichuan Panzhihua 617000，China;2.Department of Neurotumor Chemotherapy，Sanbo Brain Hospital，Capital Medical University，Beijing 100093，China;3.Department of Pathology，Panzhihua Central Hospital，Sichuan Panzhihua 617000，China.
Keywords:
lncRNA LUADT1; miR-138-5p; glioma; proliferation; migration; invasion
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2021.03.002
Abstract:
Objective：To investigate the effects and molecular mechanisms of lung adenocarcinoma associated transcript 1 (LUADT1) on the proliferation，migration and invasion of glioma cells.Methods：si-NC，si-LUADT1，miR-NC，miR-138-5p，pcDNA and pcDNA-LUADT1 were transfected into U251 cells，and record as si-NC group，si-LUADT1 group，miR-NC group，miR-138-5p group，pcDNA group and pcDNA-LUADT1 group.si-LUADT1 co-transfect with anti-miR-NC and anti-miR-138-5p into U251 cells and record as si-LUADT1+anti-miR-NC group，si-LUADT1+anti-miR-138-5p group.Real-time fluorescence quantitative PCR (RT-qPCR) detected the expressions of LUADT1 and miR-138-5p.Cell counting kit 8 (CCK-8) detected cell proliferation activity.Transwell detected cell migration and invasion.Western blot detected CyclinD1，cyclin-dependent kinase inhibitor 1A (p21)，matrix metalloproteinase 2(MMP-2)，matrix metalloproteinase 9(MMP-9) protein expression.Dual luciferase reporting assay detected targeting relationship between LUADT1 and miR-138-5p.Results：LUADT1 was highly expressed in glioma tissues and miR-138-5p was low expressed.After inhibition of LUADT1 expression or overexpression of miR-138-5p，cell viability was reduced，cell migration and invasion was reduced，CyclinD1，MMP-2，MMP-9 expressions were decreased，and p21 expression was increased.LUADT1 targeted miR-138-5p，and interfering with miR-138-5p expression reversed the inhibition of lncRNA LUADT1 expression on glioma U251 cell proliferation，migration，and invasion.Conclusion：Inhibiting the expression of LUADT1 can inhibit the proliferation，migration and invasion of glioma cells by targeting miR-138-5p.

References:

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Last Update: 2020-12-31