|Table of Contents|

Effect of hederagenin on the cell cycle of osteosarcoma blocking the endoplasmic reticulum related pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
917-921
Research Field:
Publishing date:

Info

Title:
Effect of hederagenin on the cell cycle of osteosarcoma blocking the endoplasmic reticulum related pathway
Author(s):
Huang Ying1; Wang Lihui1; Xie Xuan1; Ji Ming1; Sun Ping2; Ma Yi1; Luo Chenghong1; Li Dan1
1.Joint Surgery;2.Emergency Surgery,Central Hospital of Chongqing Three Gorges,Chongqing 404000,China.
Keywords:
osteosarcoma; apoptosis; cell cycle; hederagenin
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2020.06.010
Abstract:
Objective：To study the effect and its mechanism of hederagenin on the cell cycle of osteosarcoma.Methods:The osteosarcoma cells were treated with hederagenin of 0,5,10,20 and 40 μg/ml.CCK-8 assay was used to detect cell proliferation.Cell cloning assay was used to determine the ability of cell clone formation.The half inhibitory concentration was calculated.The osteosarcoma cells were treated with a half inhibitory concentration of hederagenin.Cell cycle and apoptosis were detected by flow cytometry,The intracellular Ca2+concentration was measured by fluorescence probe.The levels of BIP,Erol-L alpha,PDI,Cleaved Caspase-12 and protein in the cells were detected by Western blot.Results:The hederagenin of 5,10,20,40 μg/ml could inhibit the proliferation and cloning of osteosarcoma cells.Compared with 0 μg/ml hederagenin,the difference was statistically significant（P＜0.05).The proportion of G1 phase in the osteosarcoma cells treated by a half inhibitory concentration of hederagenin was increased.The rate of apoptosis was also increased,the concentration of Ca2+ in cells increased.The levels of BIP,Erol-Lα,PDI and Cleaved Caspase-12 were increased in the cells,the level of Cyclin D1 protein was reduced.Compared with 0 μg/ml hederagenin,the difference was statistically significant（P＜0.05).Conclusion:Hederagenin blocks cell cycle in G1 phase,and induces apoptosis by the endoplasmic reticulum related pathway.

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Last Update: 2020-01-21