|Table of Contents|

Effect of down-regulation of ETS1 on invasion of bladder cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
913-917
Research Field:
Publishing date:

Info

Title:
Effect of down-regulation of ETS1 on invasion of bladder cancer cells
Author(s):
Liu Lanxing; Liu Lantao
Department of Urology,the First Hospital of Weinan City,Shaanxi Weinan 714000,China.
Keywords:
bladder cancer; ETS1; MMP2; MMP9
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2020.06.009
Abstract:
Objective：To investigate the expression of ETS1 in bladder cancer（BCa) and its effect on invasion of T24 cell line.Methods:Immunohistochemical staining method was used to evaluate the expression of ETS1 protein in human normal bladder epithelia and BCa tissues which were from the department of urology of our hospital.In vitro,siRNA strategy was used to knock down ETS1 in T24 cell lines which was divided into NC（negative control) group,siETS1-1(knockdown 1) group and siETS1-2（knockdown 2) group.Transwell assay was performed to explore invasion ability of NC group,siETS1-1 group and siETS1-2 group.Western blot analysis and real-time quantitative PCR were used to detect the protein and mRNA expression of ETS1,MMP2 and MMP9 in NC group,siETS1-1 group and siETS1-2 group.Pearson correlation was used to analyze the correlation between ETS1 mRNA and MMP2 mRNA or MMP9 mRNA in BCa of TCGA database.Results:The expression of ETS1 protein was significant higher in BCa tissues than that in normal bladder epithelium ［(5.52±0.39) vs （3.36±0.53)］（P＜0.01).Real-time quantitative PCR showed that the expression of ETS1 were 1.00±0.05,0.32±0.04 and 0.28±0.02,respectively in NC group,siETS1-1 group and siETS1-2 group after transfection,and there were statistical differences among the three groups（P＜0.05).The results of Transwell showed that the invasion cells were 25.67±3.48,12.67±1.86 and 9.67±2.60,respectively in NC group,siETS1-1 group and siETS1-2 group,and there were statistical differences among the three groups（P＜0.05).The results of Western blot showed that the expression of MMP2 and MMP9 were significant lower in siETS1-1 and siETS1-2 group than NC group.The Pearson correlation showed that ETS1 was positively correlated with MMP2（P＜0.000 1) and MMP9（P＜0.000 1) in BCa.Conclusion:ETS1 was upregulated in BCa tissues and downregulation of ETS1 could inhibit BCa cell invasion.

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Last Update: 2020-01-21