|Table of Contents|

Molecular mechanism of miR-21 regulating invasion and migration of breast cancer cells via targeting ZNF326 gene

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
906-912
Research Field:
Publishing date:

Info

Title:
Molecular mechanism of miR-21 regulating invasion and migration of breast cancer cells via targeting ZNF326 gene
Author(s):
Xue Jinhui; He Yingying; Liu Ruihan; Xiao Yanjing; Zhao Na; Zhang Quanwu
Department of Pathology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Henan Zhengzhou 450000,China.
Keywords:
miR-21; ZNF326; breast cancer; proliferation; migration; invasion
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2020.06.008
Abstract:
Objective：To investigate the effect of microRNA-21（miR-21) targeting zinc finger protein 326（ZNF326) on invasion and migration of breast cancer cells and its molecular mechanism.Methods：The expressions of miR-21 and ZNF326 in breast cancer cell lines HCC70,MDA-MB-231,BT549,MDA-MB-468 and normal cell line HBL-100 were detected by qRT-PCR and Western blot.The breast cancer HCC70 cells with the highest expression of miR-21 were divided into NC group,anti-miR-con group,anti-miR-21 group,pcDNA-control group and pcDNA-ZNF326 group.The co-transfection group was anti-miR-21+si-con group,anti-miR-21+si-ZNF326 group.Cell proliferation was detected by MTT assay,and cell migration and invasion were detected by Transwell migration and invasion assay.The dual luciferase reporter gene system verified the targeted regulatory relationship between miR-21 and ZNF326.Western blot was used to detect the expression of CDK1 and MMP-2 proteins.Results:Compared with the normal cell line HBL-100,miR-21 was highly expressed in breast cancer cell lines HCC70,MDA-MB-231,BT549 and MDA-MB-468,and the expression of ZNF326 mRNA and protein was significantly decreased.Compared with anti-miR-con group and pcDNA-control group,the proliferation of HCC70 cells in anti-miR-21 group and pcDNA-ZNF326 group was significantly decreased,cell migration and invasion ability were significantly decreased,and the expression level of CDK1 and MMP-2 were significantly decreased.Dual luciferase assays showed that miR-21 could target ZNF326 and regulated its expression.Compared with the anti-miR-21+si-con group,the proliferation,migration and invasion of HCC70 cells in the anti-miR-21+si-ZNF326 group were significantly enhanced,and the expression of CDK1 and MMP-2 was promoted.Conclusion:miR-21 can target the inhibition of ZNF326 gene expression and promote breast cancer cell proliferation,migration and invasion.

References:

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Last Update: 2020-01-21