|Table of Contents|

Experimental study on the inhibitory effect of miR-29a regulatory CLDN1 on the proliferation of hepatocellular carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
887-891
Research Field:
Publishing date:

Info

Title:
Experimental study on the inhibitory effect of miR-29a regulatory CLDN1 on the proliferation of hepatocellular carcinoma cells
Author(s):
Shaya·Mahati; Dina·Ainiwaer; Xiao Lei; Zhang Hua
Department of Tumor Center,First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830011,China.
Keywords:
hepatocellular carcinoma; miR-29a; proliferation; migration; CLDN1
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2020.06.004
Abstract:
Objective：To explore the expression level of miR-29a in liver cancer tissue and its effect on the proliferation ability of human liver cancer cells.Methods:The expression of miR-29a in hepatocellular carcinoma and non-hepatocellular carcinoma tissues were detected by RT-PCR.miR-29a mimics,empty vector miR-29a-NC and miR-29a inhibitors were transfected into hepatocellular carcinoma HepG2 and HLE cell lines, RT-PCR methods were used to observe the effects of miR-29a on proliferation of hepatocellular carcinoma cell lines HepG2 and HLE. The target genes of miR-29a were predicted by Targetscan software and verified by Western blot and luciferase experiments.Results:Compared with non-hepatocarcinoma tissues,miR-29a was down-regulated in hepatocarcinoma tissues.MTT experiments showed that the proliferation ability of hepatoma cells in the high expression group of miR-29a were weak.The proliferation ability of hepatoma cells in the low expression group of miR-29a were increased.Targetscan software suggested that CLDN1 may be a target gene of miR-29a.Western blot and luciferase assays showed that the expression of CLDN1 was decreased in the high expression group of miR-29a compared with the negative control group,and the expression of CLDN1 was decreased in the low expression group of miR-29a.Conclusion:miR-29a is down-regulated in liver cancer cells and tissues,and may affect the proliferation of liver cancer cells by regulating CLDN1.

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Memo

Memo:
新疆维吾尔自治区自然科学基金（编号：2015211C079）
Last Update: 2020-01-21