|Table of Contents|

miR-106a inhibits the proliferation of osteosarcoma cells via downregulation of MAP3K2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
883-887
Research Field:
Publishing date:

Info

Title:
miR-106a inhibits the proliferation of osteosarcoma cells via downregulation of MAP3K2
Author(s):
Cheng Jie1; Ke Wentan1; Li Jingyuan2
1.Department of Orthopaedics,Huangshi Aikang Hospital,Hubei Huangshi 435000,China;2.Department of Orthopaedics,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China.
Keywords:
microRNA-106a; osteosarcoma cells; proliferation; MAP3K2
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2020.06.003
Abstract:
Objective：To investigate the effect and mechanism of miR-106a on proliferation of osteosarcoma cells.Methods:Real-time PCR was performed to detect the expression of miR-106a in normal osteoblasts（hFOB11.9) and three types of osteosarcoma cells（MG-63,U-2OS and HOS).Then,negative control（NC),miR-106a mimics and miR-106a inhibitor were transfected into MG-63 cells respectively.After transfection,CCK-8 and colony formation assays were constructed to detect the effect of miR-106a on cell proliferation.Mitogen-activated protein kinase kinase kinase 2（MAP3K2) was predicted as a target of miR-106a by bioinformatics software.The vectors of wild-type and mutant MAP3K2 3'-UTR binding zone were then constructed.And the regulation of MAP3K2 by miR-106a was illuminated by luciferase reporter assay.Western blot was used to detect the protein level of MAP3K2 in MG-63 cells transfected with NC and miR-106a mimics.Results:Real-time PCR indicated that,compared with normal osteoblasts,the expressions of miR-106a in osteosarcoma cells MG-63,U-2OS and HOS were significantly reduced（P<0.05).The growth curve of CCK-8 and colony formation assay showed that miR-106a significantly inhibited the growth of MG-63（P＜0.05) compared with NC,while miR-106a inhibitor promoted the growth of tumor cells（P＜0.05).The luciferase reporter assay indicated that the luciferase activity of wild type MAP3K2 3'-UTR was significantly inhibited by the miR-106a mimics（P＜0.05),while there was no significant difference between the control and the MAP3K2 3'-UTR mutant group（P=0.877).Compared with NC group,MAP3K2 expression was significantly reduced in miR-106a mimics transfection group（P＜0.05).Conclusion:miR-106a may inhibit the growth of osteosarcoma cells via down-regulating the expression of MAP3K2.

References:

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Memo

Memo:
陕西省自然科学基金项目（编号：2017JQ8030）
Last Update: 2020-01-21