|Table of Contents|

Effects of siRNA targeting silencing MALAT-1 on proliferation,apoptosis and PI3K/ATK pathway of nasopharyngeal carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 06
Page:
892-896
Research Field:
Publishing date:

Info

Title:
Effects of siRNA targeting silencing MALAT-1 on proliferation,apoptosis and PI3K/ATK pathway of nasopharyngeal carcinoma cells
Author(s):
Cao Yali; Li Honghui; Shao Yuan; Zhu Yun; Huang Jianhua; Zhang Pengfei
Department of Otolaryngology,Head and Neck Surgery,First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.
Keywords:
siRNA silencing; proliferation; apoptosis; PI3K/AKT pathway; MALAT-1; nasopharyngeal carcinoma cells
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2020.06.005
Abstract:
Objective：To investigate the effects of siRNA targeting silencing MALAT-1 on proliferation,apoptosis and PI3K/AKT pathway of nasopharyngeal carcinoma cells.Methods:The MALAT-1 gene of human nasopharyngeal carcinoma cell line CNE was targeted silenced with siRNA at concentrations of 10,20,30,40 and 50 nmol/L,respectively.Real-time fluorescence quantitative analysis（qRT-PCR) was used to detect the expression of MALAT-1 after 24 hours and 48 hours respectively,screened suitable concentration and time of siRNA treatment.CNE cells were divided into three groups:Blank control group,siRNA negative control group and siRNA group.After siRNA treatment of cells,CCK-8 method was used to detect cell proliferation.Cell apoptosis was detected by flow cytometry.Western blot was used to detect the expressions of apoptosis-related proteins（Bcl-2,Bax,caspase-3),PI3K/AKT pathway proteins（PI3K,ATK,p-AKT).Results:30 nmol/L siRNA was selected to act on CNE cells for 48 hours.Compared with the blank control group,the proliferation rate of cells treated with siRNA decreased significantly.The apoptotic rate and the expressions of Bax and caspase-3 proteins were significantly increased.The expressions of Bcl-2,PI3K and p-AKT proteins were significantly decreased（P<0.05).There was no significant change in AKT protein expression（P>0.05).There was no significant difference in the indexes of siRNA negative control group（P>0.05).Conclusion:siRNA can silence MALAT-1,inhibit the proliferation and promote apoptosis of nasopharyngeal carcinoma cells,probably by inhibiting PI3K/AKT pathway.

References:

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Memo

Memo:
陕西省自然科学基础研究计划（面上项目）（编号：2017JM8072）
Last Update: 2020-01-21