|Table of Contents|

Effect of β-Lapachone on the proliferation，migration and apoptosis ability of breast cancer cells and mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
751-755
Research Field:
Publishing date:

Info

Title:
Effect of β-Lapachone on the proliferation，migration and apoptosis ability of breast cancer cells and mechanism
Author(s):
Wang Wei1; Zhang Hai2; Luo Chao1; Hu Xuan1
1.Breast Department；2.Pathology Department，Panzhihua Maternal and Child Health Hospital，Sichuan Panzhihua 617000，China.
Keywords:
breast cancer; β-Lapachone; proliferation; migration; cell apoptosis
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2020.05.012
Abstract:
Objective：To investigate the effects of β-Lapachone on proliferation，migration and apoptosis in breast cancer cells (MCF-7 and MFM223) and its mechanism in vitro．Methods：The cell viability was detected using MTT，colony formation assay.The migration ability was determined using scratch assay method，and the apoptosis was examined using flowcytometry.Meanwhile，the expression of biomarkers of proliferation，EMT markers and apoptosis were detected using Western blot analysis．Results：β-Lapachone could significantly inhibit the proliferation of MCF-7 and MFM223 breast cancer cells (P<0.05).β-Lapachone could also inhibit the migration and motility of breast cancer cells via down-regulating the expression levels of MMP-2/9 and Ezrin proteins and up-regulating the epithelial markers.In addition，β-Lapachone enhanced the apoptosis of breast cancer cells，down-regulated BCL-2/Bax ratio and up-regulated of activated Caspase-3/8/9.Conclusion：β-Lapachone can effectively inhibit the proliferation and induce the apoptosis of breast cancer cells，and inhibit the migration of gastric cancer cells via MMPs and EMT pathways．

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Memo

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四川省医学会科研项目（编号：2015SHD014）
Last Update: 1900-01-01