|Table of Contents|

Study on the apoptosis of osteosarcoma cells induced by mitochondrial pathway by PUMA

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
746-750
Research Field:
Publishing date:

Info

Title:
Study on the apoptosis of osteosarcoma cells induced by mitochondrial pathway by PUMA
Author(s):
Huang Ying1; Wang Lihui1; Ji Ming1; Sun Ping2; Ma Yi1; Luo Chenghong1; Li Dan1
1.Joint Surgery；2.Emergency Surgery，Central Hospital of Chongqing Three Gorges，Chongqing 404000,China.
Keywords:
osteosarcoma; mitochondrial pathway; apoptosis; PUMA
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2020.05.011
Abstract:
Objective：To study the effect of PUMA on the apoptosis of osteosarcoma cells and its mechanism.Methods：PUMA overexpression vector in osteosarcoma cell F5M2 was transfected,at the same time，the control negative vector was transfected.The expression of PUMA in the transfected cells was measured by qRT-PCR and Western blot.Cell apoptosis was detected by flow cytometry.The JC-1 method was used to detect the mitochondrial membrane potential.The expression of Cyt C protein in mitochondria and cytoplasm was measured by Western blot method.The levels of Cleaved Caspase-3 and Cleaved Caspase-9 protein were also detected.Results：The level of PUMA mRNA and protein increased in the F5M2 cells transfected with PUMA overexpression vector，the transfection control negative carrier had no effect on the level of PUMA mRNA and protein in F5M2 cells.The apoptosis rate of F5M2 cells was increased after overexpression of PUMA.The membrane potential of cell mitochondria was decreased.The level of Cyt C protein in mitochondria was reduced，and the level of Cyt C protein in the cytoplasm was increased.At the same time，the level of Cleaved Caspase-3 and Cleaved Caspase-9 protein in the cells was also increased.Compared with the F5M2 cells that were not transfected，the difference was statistically significant (P<0.05).The apoptotic rate，mitochondrial membrane potential，mitochondria and cytoplasmic Cyt C protein level，Cleaved Caspase-3 protein level and Cleaved Caspase-9 protein level of transfected control negative vectors were not significantly different from those of F5M2 cells without transfection (P>0.05).Conclusion：PUMA reduces mitochondrial membrane potential by promoting the release of Cyt C in mitochondria and activates Caspase-3 to induce apoptosis of osteosarcoma cells.

References:

［1］Yao W，Cai Q，Wang J，et al.Treatment of osteosarcoma around the knee in skeletally immature patients［J］.Oncology Letters，2017，14(5)：5241-5248.
［2］Zou Y，Yang J，Wu J，et al.miR-133b induces chemoresistance of osteosarcoma cells to cisplatin treatment by promoting cell death，migration and invasion［J］.Oncology Letters，2018，15(1)：1097-1102.
［3］Yang S，Zhu Z，Zhang X，et al.Idelalisib induces PUMA-dependent apoptosis in colon cancer cells［J］.Oncotarget，2017，8(4)：6102-6113.
［4］Hao C，Wang H，Xin H，et al.Targeting NF-κB and PUMA prevents heart injury in heart transplantation［J］.International Journal of Clinical & Experimental Pathology，2017，10(5)：5765-5771.
［5］Dikmen M，Kaya-Tilki E，Engur S，et al.Neuritogenic activity of epigallocatechin gallate and curcumin combination on rat adrenal pheochromocytoma cells［J］.Fresenius Environmental Bulletin，2017，26(7)：4726-4733.
［6］Azizizadeh H，Najafpour A.Interaperitoneal administration of αlpha-tocopherol loaded nanoparticles improves ischemia-reperfusion injury in rat ovaries torsion and detorsion model［J］.Bulletin of Emergency & Trauma，2018，6(3)：207-216.
［7］Dodo K，Shimizu T，Sasamori J，et al.Indolylmaleimide derivative IM-17 shows cardioprotective effects in ischemia-reperfusion injury［J］.Acs Medicinal Chemistry Letters，2018，9(3)：182-187.
［8］Yang J，Zhao X，Tang M，et al.The role of ROS and subsequent DNA-damage response in PUMA-induced apoptosis of ovarian cancer cells［J］.Oncotarget，2017，8(14)：23492-23506.
［9］Zhang L，Wang H，Li W，et al.Pazopanib，a novel multi-kinase inhibitor，shows potent antitumor activity in colon cancer through PUMA-mediated apoptosis［J］.Oncotarget，2017，8(2)：3289-3303.
［10］Sun CG，Zhuang J，Teng WJ，et al.PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells［J］.Genetics & Molecular Research Gmr，2015，14(2)：5742.
［11］YANG T，ZHANG JY，CEN YZ，et al.The effect of PUMA gene on the apoptosis of breast cancer cells induced by silybin［J］.Chinese Maternal and Child Health Care，2013，28(36)：6044-6046.［羊涛，张嘉越，岑延增，等.PUMA基因在水飞蓟宾诱导乳腺癌细胞凋亡的作用［J］.中国妇幼保健，2013，28(36)：6044-6046.］
［12］Karakas B，Ozmay Y，Basaga H，et al.Distinct apoptotic blocks mediate resistance to panHER inhibitors in HER2+ breast cancer cells［J］.Biochimica et Biophysica Acta (BBA)-Molecular Cell Research，2018，1865(8)：1073-1087.
［13］Huun J，Gansmo LB，Mannsker B，et al.The functional roles of the MDM2 splice variants P2-MDM2-10 and MDM2-△5 in breast cancer cells［J］.Translational Oncology，2017，10(5)：806-817.
［14］Figueroa CM，Suarez BN，Molina AM，et al.Smart release nano-formulation of cytochrome c and hyaluronic acid induces apoptosis in cancer cells［J］.Journal of Nanomedicine & Nanotechnology，2017，8(1)：2180-2189.
［15］Wang YY，Yuk-Kwan Chen DDS MS，Ya-Ling Hsu MS，et al.Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction［J］.Head & Neck，2017，39(6)：1055-1064.
［16］Wang YY，Chen YK，Hu SC，et al.CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis［J］.Cancer Chemotherapy & Pharmacology，2017，79(6)：1-12.
［17］Khorobrykh AA，Yanykin DV，Klimov VV.Photooxidation and photoreduction of exogenous cytochrome c，by photosystem II preparations after various modifications of the water-oxidizing complex［J］.Photosynthetica，2018，56(1)：1-10.
［18］Paul PK，Che DC，Hiroyuki K，et al.Adsorption characteristics of Cytochrome c/DNA complex Langmuir molecular assemblies at the air-water interface：A surface area-normalized isotherm study［J］.Rsc Advances，2017，7(60)：37755-37764.
［19］Li Z，Guan P，Hu X，et al.Preparation of molecularly imprinted mesoporous materials for highly enhancing adsorption performance of cytochrome C［J］.Polymers，2018，10(3)：298.
［20］Liu W，Li H，Wei Y，et al.A label-free phosphorescence sensing platform for trypsin based on Mn-ZnS QDs［J］.Rsc Advances，2017，7(43)：26930-26934.
［21］Porcelli L，Iacobazzi RM，Quatrale AE，et al.Grape seed extracts modify the outcome of oxaliplatin in colon cancer cells by interfering with cellular mechanisms of drug cytotoxicity［J］.Oncotarget，2017，8(31)：50845-50863.
［22］Lv R，Du L，Lu C，et al.Allicin protects against H2O2-induced apoptosis of PC12 cells via the mitochondrial pathway［J］.Experimental & Therapeutic Medicine，2017，14(3)：2053-2059.
［23］Huang L，Li A，Liao G，et al.Curcumol triggers apoptosis of p53 mutant triple-negative human breast cancer MDA-MB 231 cells via activation of p73 and PUMA［J］.Oncology Letters，2017，14(1)：1080.
［24］Gilardini Montani MS，Granato M，Santoni C，et al.Histone deacetylase inhibitors VPA and TSA induce apoptosis and autophagy in pancreatic cancer cells［J］.Cellular Oncology，2017，40(2)：1-14.
［25］Wang J，Guo X，Xie C，et al.KIF15 promotes pancreatic cancer proliferation via the MEK-ERK signalling pathway［J］.British Journal of Cancer，2017，117(2)：245-255.

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