|Table of Contents|

miR-214 inhibits migration and invasion of myeloma cells by targeting MCL-1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
740-746
Research Field:
Publishing date:

Info

Title:
miR-214 inhibits migration and invasion of myeloma cells by targeting MCL-1
Author(s):
Sun FeiWang ShuoZhao Qian
Department of Orthopaedics,Zaozhuang Mining Group Zaozhuang Hospital,Shandong Zaozhuang 277100,China.
Keywords:
myelomamiR-214MCL-1migrationinvasion
PACS:
R733.3
DOI:
10.3969/j.issn.1672-4992.2020.05.010
Abstract:
Objective:To clarify the relationship between microRNAs-214 and MCL-1 and their effects on the invasion and migration of myeloma cells.Methods:qRT-PCR and Western blot were used to detect the expression of miR-214 and MCL-1 in myeloma cells and analyze the correlation.Transwell cell assay was used to detect the effects of overexpression of miR-214 or silencing of MCL-1 on migration and invasion of myeloma cell lines H929 and U266.Luciferase reporter gene assay confirmed the target relationship between miR-214 and MCL-1.Western blot detected the effect of up regulation or down regulation of miR-214 on MCL-1 expression.Results:The expression of miR-214 was downregulated in myeloma cells,while MCL-1 was upregulated,and the two were negatively correlated.Up regulation of miR-214 or knockdown of MCL-1 significantly inhibited the invasion and migration of H929 and U266 in myeloma cells.Luciferase reporter gene assay confirmed that MCL-1 was the target gene of microRNAs-214.Western blot showed that miR-214 negatively regulated the expression of MCL-1.Additional supplementation of MCL-1 reversed the inhibitory effect of miR-214 on migration and invasion of myeloma cells H929 and U266.Conclusion:miR-214 inhibits migration and invasion of myeloma cells through negative regulation of MCL-1 expression.

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