|Table of Contents|

miRNA-1 inhibits proliferation，migration，and invasion of esophageal cancer cells by targeting DDX5

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
702-705
Research Field:
Publishing date:

Info

Title:
miRNA-1 inhibits proliferation，migration，and invasion of esophageal cancer cells by targeting DDX5
Author(s):
Cao Qiong1; Dai Aijun1; Li Yichuan2; Chang Jingzhi2
1.Department of Pathology，the Third Affiliated Hospital of Henan University of Science and Technology，Luoyang Dongfang Hospital,Henan Luoyang 471003，China；2.Department of Biochemistry and Molecular Biology，Shangqiu Medical College，Henan Shangqiu 476100，China.
Keywords:
miR-1; esophageal cancer; DDX5; proliferation; migration
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2020.05.003
Abstract:
Objective：To investigate the effects of microRNA (miR)-1 on growth，invasion and migration of esophageal cancer cell line EC-109 and its related regulatory mechanisms.Methods：miR-1 mimics were transfected into EC-109 cells in vitro using liposome transient transfection.CCK-8 assay and Transwell assay were used to detect the proliferation，invasion and migration of esophageal cancer cells.Bioinformatics predicted the potential target genes of miR-1，and dual-luciferase reporter gene and Western blot were used to validate it.Results：After over-expression of miRNA-1，CCK-8 and Transwell assays showed that the proliferation，invasion and migration of esophageal cancer cells were significantly decreased (P<0.05).Bioinformatics analysis showed that DDX5 may be the target gene of miR-1.The dual-luciferase reporter results showed that miR-1 bound to the DDX5 mRNA 3' UTR.Western blot results showed that the expression of DDX5 decreased after high expression of miR-1.Conclusion：miRNA-1 may inhibit the proliferation，migration and invasion of esophageal cancer cells by negatively-regulated expression of DDX5.

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