|Table of Contents|

High expression of hnRNP K protein in gastric cancer cells regulates the expression of miR-20a

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
706-712
Research Field:
Publishing date:

Info

Title:
High expression of hnRNP K protein in gastric cancer cells regulates the expression of miR-20a
Author(s):
Fang Haiyun12Guo Jiahui1Gao Fenghou1
1.Department of Oncology,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 201900,China; 2.Department of Laboratory Medicine,Xinhua Hospital Chongming Branch,Shanghai Jiaotong University School of Medicine,Shanghai 202150,China.
Keywords:
gastric cancerhnRNP KmicroRNAchipTCGA databasemiR-20a
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.05.004
Abstract:
Objective:To explore the expression of microRNAs directly regulated by hnRNP K protein in gastric cancer cells.Methods:The microRNA expression profiles of gastric cancer cells with high expression of hnRNP K were screened by cell microarray of normal gastric mucous membrane cell GES-1 and gastric cancer cell line SGC-7901.Large samples of transcription group and microRNA data were obtained by querying TCGA database,and the microRNA differential expression profiles corresponding to hnRNP K with high expression of 2-fold difference were obtained by analysis and processing.The co-expression of microRNAs was up-regulated by interactive analysis of the results of microarray and TCGA database.hnRNP K was overexpressed or knocked down and the results of screening were validated by Western Blot and Real Time q-PCR.hnRNP K and microRNAs were normalized with TCGA data to verify the correlation between the screened microRNAs and hnRNP K in gastric cancer.Results:The co-expression profile of miR-17/20a/24/182 was up-regulated by microarray screening and TCGA query results.Western Blot and Real Time q-PCR results showed that hnRNP K could directly regulate the expression of miR-20a.TCGA data analysis showed that miR-20a was positively correlated with hnRNP K and highly expressed in gastric cancer.Conclusion:High expression of HnRNP K protein in gastric cancer cells up-regulates the expression of miR-20a.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.81172322);国家自然科学基金(编号:81172322)
Last Update: 1900-01-01